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| 实验性大鼠弥漫性轴索损伤的傅里叶红外光谱检测 | |
| 引用本文： | 杨天潼,何光龙,孙婷怡,张海东,曹洪林,李玲.实验性大鼠弥漫性轴索损伤的傅里叶红外光谱检测[J].中国法医学杂志,2012,27(5):353-355,432. |
| 作者姓名： | 杨天潼 何光龙 孙婷怡 张海东 曹洪林 李玲 |
| 作者单位： | 1. 中国政法大学教育部证据科学重点实验室,北京,100040 2. 公安部物证鉴定中心,北京,100038 3. 华中科技大学同济医学院,湖北武汉,430030 4. 中国政法大学教育部证据科学重点实验室,北京100040;华中科技大学同济医学院,湖北武汉430030;美国马里兰州法医局,巴尔的摩21223 |
| 基金项目： | 国家自然科学基金青年科学基金项目(81001349);中国政法大学青年教师学术创新团队资助项目 |
| 摘 要： | 目的探讨利用傅里叶红外光谱技术诊断弥漫性轴索损伤的可行性。方法利用HE染色、银染和β-APP免疫组化染色确认大鼠弥漫性轴索损伤模型,利用傅里叶变换显微红外光谱面扫描成像技术检测弥漫性轴索损伤区域酰胺Ⅱ带的光谱分布情况,得到弥漫性轴索损伤的红外光谱数据,绘制红外光谱病理图像。结果实验组与对照组的酰胺Ⅱ带红外光谱吸收度之间存在明显差异,红外光谱病理图像中酰胺Ⅱ带的高吸收区与弥漫性轴索损伤区符合。结论傅里叶红外光谱技术可对弥漫性轴索损伤进行病理形态学诊断。 |
| 关 键 词： | 法医病理学 弥漫性轴索损伤 傅里叶红外光谱 |
Primary study on diffuse axonal injury by FTIR spectral histopathology imaging |
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| Yang Tiantong , He Guanglong , Sun Tingyi , Zhang Haidong , Cao Honglin , Li Ling.Primary study on diffuse axonal injury by FTIR spectral histopathology imaging[J].Chinese Journal of Forensic Medicine,2012,27(5):353-355,432. | |
| Authors: | Yang Tiantong He Guanglong Sun Tingyi Zhang Haidong Cao Honglin Li Ling |
| Institution: | 1,3,4/1.China University of Political Science and Law,Key Laboratory of Evidence Science,Ministry of Education,Beijing 100040,China;2.Institute of Forensic Science of Ministry of Public Security,Beijing 100038,China;3.Faculty of Forensic Medicine,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;4.The Office of Chief Medical Examiner,Maryland 21223,USA) |
| Abstract: | Objective To discuss a new method for diffuse axonal injury(DAI) diagnosis by fourier transform infrared spectroscopy(FTIR).Methods The animal models of DAI were established.HE stain,Sliver stain,β-APP stain and infrared spectroscopy detection were conducted on the rat brains with DAI.Infrared spectroscopy mapping was performed to determine Amide Ⅱ stretch chemical distribution,obtained infrared data and FTIR-mapping of DAI.Results FTIR data showed a significant difference between experimental group and control group.A high absorbance of Amide Ⅱ matched with DAI regions.Conclusion These findings suggest that FTIR may be used for pathological diagnosis of diffuse axonal injury. |
| Keywords: | forensic pathology fourier transform infrared spectroscopy diffuse axonal injury |
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