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| 液相色谱/质谱法检测血液中的艾司佐匹克隆 | |
| 引用本文： | 张蕾萍,周红,张榆梓,舒翠霞.液相色谱/质谱法检测血液中的艾司佐匹克隆[J].中国法医学杂志,2012,27(3):228-230. |
| 作者姓名： | 张蕾萍 周红 张榆梓 舒翠霞 |
| 作者单位： | 1. 公安部物证鉴定中心,北京,100038 2. 中国人民公安大学,北京,100038 3. 中国医科大学,辽宁沈阳,110001 |
| 基金项目： | 中央公益性基金项目(2010JB001) |
| 摘 要： | 目的建立手性分离-液相色谱/质谱法,用于分离检测血液中艾司佐匹克隆。方法血液经HLB小柱固相萃取后,采用手性分离柱,用电喷雾正离子模式离子化、多反应监测模式检测艾司佐匹克隆,外标法定量。结果采用本文方法可有效分离佐匹克隆的左旋、右旋异构体,血中艾司佐匹克隆在1～100ng/mL范围内线性关系良好,相关系数为0.996 4,最低检出限为0.8ng/mL,回收率为76%～88%,日内与日间精密度均小于8%。结论本文所建方法简便、快速、分离度好,适用于血液中艾司佐匹克隆的检测。 |
| 关 键 词： | 法医毒物分析 艾司佐匹克隆 佐匹克隆 手性分离 液相色谱/质谱 |
Deternination of eszopiclone in blood by liquid chromatography-tandem mass spectrometry |
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| Zhang Leiping , Zhou Hong , Zhang Yuzi , Shu Cuixia.Deternination of eszopiclone in blood by liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Forensic Medicine,2012,27(3):228-230. | |
| Authors: | Zhang Leiping Zhou Hong Zhang Yuzi Shu Cuixia |
| Institution: | 1.Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China;2.Chinese People’s Public Security University,Beijing 100038,China;3.China Medical University,Shenyang 110001,China) |
| Abstract: | Objective To establish a method of chiral separation-liquid chromatography/tandem mass spectrometry for determination of eszopiclone in blood.Methods blood sample was extracted using HLB solid phase extraction.The extract was separated on chiral separation column,confirmed and quantified by MS-MS in the multiple reaction monitoring(MRM) mode via positive electrospray ionization mode.Results Eszopiclone in blood was separated well.Calibration curves were linear within the range of 1~100ng/mL(R2=0.996 4),and the limits of detection were 0.8ng/mL.The recoveries were between 76%~88%,the inter-day and intra-day precision were less than 8%.Conclusion This method is fast and good resolution and suitable for determination of trace eszopiclone in blood. |
| Keywords: | forensic toxicological analysis eszopiclone zopiclone chiral separation LC/MS |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |