大鼠皮肤挫伤修复过程中NF_κB P65表达的研究

目的探讨皮肤挫伤修复过程中,核转录因子(NFkB)P65在挫伤区不同时间表达的变化规律。方法 应用免疫组化技术对挫伤后0h、3h、6h、9h、12h、1d、3d、5d、7d、10d的大鼠皮肤挫伤组织中NFkB P65的表达进行研究。结果伤后3h,挫伤区内浸润的少量多核粒细胞的胞质中NFkB P65呈弱阳性表达,阳性率为(3.20％±0.15％);伤后6h,阳性表达增强,阳性率为(11.33％±0.88％);伤后12h,表达继续增强,阳性率(75.67％±0.82％);伤后1d,在浸润的几乎所有单核细胞和多核粒细胞的胞质和胞核中均可见NFkB P65呈强阳性表达,阳性率为(97.33％±0.88％),表达达高峰,以后逐渐下降。结论 大鼠皮肤挫伤修复过程中,NFkB P65在挫伤后的炎症反应中发挥重要作用;同时,NFkB P65在挫伤区内多核粒细胞,单核细胞及成纤维细胞中的表达呈规律性变化,可对挫伤时间的推断提供参考。

Study on the Expression of NFkB P65 During Contusion Repair in the Rat Skin

Objective To investigate the expression of NFkB P65 during rat skin contusion repair. Methods NFkB P65 was studied using Immunohistochemical technique at 0, 3 , 6, 9, 12 hours and 1, 3 , 5 , 7 , 10 days postcontusion. Results On postcontusion hour 3 , weak expression of NFkB P65 was detected in cy-toplasma of polymorphonuclear cells (PMNs) that infiltrated in to the injured area, the ratio of the NFkB P65 positive cells was low (3. 20%±0. 15% ). On postcontusion hours 6 to 12, the expression of NFkB P65 was prominently increased, the ratio was (11. 33%±0. 88% ) and (75. 67%±0. 82% ). On postcontusion 24 hour, almost all of the mononuclear cells ( MNCs) and PMNs were NFKB P65 positive and the cytoplasm and nucleus were all NFkB P65 stained (97.33%±0.88%). Thereafter, the expression of NFkB P65 decreased. Conclusion These results suggest that NFKB P65 may play a major role in the injured-induced in-flammatoruy response. Furthemore, NFkB P65 may be used as a marker for the contusion age determination, because NFkB P65 was time-dependently expressed in PMNs, MNCs and FBCs during skin injury repairation in rat.