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| 氯硝安定在生物体内的代谢 | |
| 引用本文： | 黄克建,李宏森,朱定姬,林翠梧,刘晓锋,李璐,罗正坚,陈而廉.氯硝安定在生物体内的代谢[J].中国法医学杂志,2009,24(5):292-295. |
| 作者姓名： | 黄克建 李宏森 朱定姬 林翠梧 刘晓锋 李璐 罗正坚 陈而廉 |
| 作者单位： | 1. 广西壮族自治区公安厅物证鉴定中心,广西,南宁,530012 2. 广西大学化学化工学院,广西,南宁,530004 3. 广西壮族自治区公安厅科技处,广西,南宁,530012 |
| 基金项目： | 公安部应用创新计划资助项目 |
| 摘 要： | 目的推测氯硝安定在生物体内的主要代谢方式,检测其代谢产物。方法取5只Wistar大鼠,连续3d给每只大鼠灌胃氯硝安定（剂量分别为1、2、2mg）,收集灌胃后24h内尿液;给3只大鼠各灌胃2mg氯硝安定,2h后处死,取血液和肝组织等检材;收集3名口服5mg氯硝安定的志愿者24h内尿液。分别对不同检材进行处理后,气相色谱/质谱联用（GC/MS）检测。结果在大鼠阳性尿液和志愿者阳性尿液中,均检出7-乙酰氨基氯硝安定和7-氨基氯硝基安定;在大鼠血液中,主要检出氯硝安定和7-氨基氯硝基安定;在大鼠肝组织中,主要检出7-乙酰氨基氯硝安定,还有少量7-氨基氯硝安定和氯硝安定。结论氯硝安定进入生物体后,其7位硝基被还原为氨基,氨基接着被乙酰化,形成7-氨基氯硝基安定和7-乙酰氨基氯硝安定代谢物,其中7-乙酰氨基氯硝安定为主要代谢物。 |
| 关 键 词： | 法医毒物分析 氯硝安定 药物代谢 气相色谱/质谱联用 |
Studies on metabolic transformation of clonazepam in the body |
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| HUANG Kejian,LI Hongsen,ZHU Dingji,LIN Cuiwu,LIU Xiaofeng,LI Lu,LOU Zhengjian,CHEN Erkang.Studies on metabolic transformation of clonazepam in the body[J].Chinese Journal of Forensic Medicine,2009,24(5):292-295. | |
| Authors: | HUANG Kejian LI Hongsen ZHU Dingji LIN Cuiwu LIU Xiaofeng LI Lu LOU Zhengjian CHEN Erkang |
| Institution: | (HUANG Kejian1,LI Hongsen1,ZHU Dingji2,LIN Cuiwu2,LIU Xiaofeng1,LI Lu1,LOU Zhengjian1,CHEN Erkang3/1.Institution of Forensic Science,Guangxi Zhuang Autonomous Region Public Security,Nanning 530012,China,2.College of Chemistry and Engineering,Guangxi university,Naning 530004,3.Department of technology,Public Security of Guangxi Zhuang Antonomous Region,China) |
| Abstract: | Objective To study the metabolites and metabolic process of clonazepam in the body.Methods Five wistar rats were gavaged(each with 1,2,2mg/d dose,respectively)for three consecutive days,collected the urine within 24h.Three wistar rats each gavaged 2mg clonazepam were killed after 2h,the blood and the liver specimens were collected.The 24 hour urine of the human volunteers after oral administration of 5mg clonazepam was also collected.After pretreatment,all the samples were analyzed by gas chromatography-mas... |
| Keywords: | Forensic toxicology analysis Clonazepam Metabolize Gas chromatography-mass spectrometry(GC/MS) |
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