| 人DNA指纹检测的初步研究 |
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| 引用本文： | 伍新尧,罗超权,杨英浩,刘照文.人DNA指纹检测的初步研究[J].中国法医学杂志,1989(4). |
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| 作者姓名： | 伍新尧 罗超权 杨英浩 刘照文 |
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| 作者单位： | 中山医科大学，中山医科大学，中山医科大学，中山医科大学 |
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| 摘 要： | 根据随机探针检测DNA限制片段长度多态性的原理和人与鼠的髓鞘硷性蛋白(MBP)基因cDNA有90％以上同源序列的事实,我们选用rMBP-cDNA 3'端非表达区高度重复顺序的0.81kb片段作探针,检测用HaeⅢ酶解的人DNA限制性片段结果可以分解出22条谱带,受检的30例无血缘关系的个体之间,没有两个人的谱带是完全相同的,显示出此方法的高度特异性。本文还比较了若干DNA片段作探针和几种限制性内切酶检测人DNA指纹的结果。
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| 关 键 词： | DNA指纹 限制性片段长度多态性 |
The STUDY OF HUMAN DNA FINGERPRINTS |
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| Wu xinyao Luo Chaoquan Yang Yinghao Liu Zhaowen Sun yat-sen University of Medical Sciences,Guangzhou.The STUDY OF HUMAN DNA FINGERPRINTS[J].Chinese Journal of Forensic Medicine,1989(4). |
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| Authors: | Wu xinyao Luo Chaoquan Yang Yinghao Liu Zhaowen Sun yat-sen University of Medical Sciences Guangzhou |
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| Abstract: | Basing on the principle of detecting restriction fragment length polymorphisms (RFLPs) With anonymous DNA probes and the fact that there were more than 90% homologeous sequences in the myelin basic protein (MBP) gene between man and rat, we used the 0. 81kb fragment in highly repeat sequence of the 3'-end non-coding region of rMBP-cDNA as a probe to detect RFJLs of human DNA digested with HaeⅢ. 22 bands were found on the autoradiographes. The identical autoradiographes between two unrelated persons was not found the DNA finger-prints obtained is highly specific. The DNA fingerprints obtained by several DNA probes and restriction endonucleases were compared. |
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| Keywords: | DNA fingerprint Restriction length polymorphismsl (RFLPs) |
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