| 错配引物诱导酶切技术检测GC多态性 |
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| 引用本文： | 林刻智,楼迪栋.错配引物诱导酶切技术检测GC多态性[J].中国法医学杂志,2009,24(5):323-325. |
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| 作者姓名： | 林刻智 楼迪栋 |
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| 作者单位： | 温州医学院法医学教研室,浙江,温州,325027 |
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| 摘 要： | 目的探讨建立检测GC点突变rs7041的引物错配诱导酶切技术（mismatch primer-induced RFLP,MPIR）及应用价值。方法针对GC基因点突变（NCBI Reference SNP ID：rs7401）,设计错配引物进行PCR扩增,引物错配碱基结合GC点突变诱导XhoI酶切,产生GC-rs7041片断长度多态性,并调查183例温州汉族无关群体GC-rs7401多态性。结果引物错配诱导酶切技术对GC-rs7041亚型的3种基因型分型明确。温州地区汉族人群GC-rs7041 T/G基因频率分别为0.787和0.213,基因型频率分别为T/T0.685、T/G 0.284和G/G 0.071,Ho（0.284）、He（0.337）、PIC（0.279）、DP（0.502）、PE（0.140）,基因型分布符合Hardy-Weinberg平衡。结论成功建立引物错配诱导酶切技术检测GC-rs7041单核苷酸多态性,该位点多态性有实际应用价值。
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| 关 键 词： | 法医物证学 型特异性成份(GC) 引物错配酶切 遗传学多态性 单核苷酸多态性 |
A study of GC-subtype polymorphism in Han population of Wenzhou city using mismatch primer-induced restriction sites |
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| LIN Kezhi,LOU Didong.A study of GC-subtype polymorphism in Han population of Wenzhou city using mismatch primer-induced restriction sites[J].Chinese Journal of Forensic Medicine,2009,24(5):323-325. |
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| Authors: | LIN Kezhi LOU Didong |
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| Institution: | (LIN Kezhi,LOU Didong/Department of Forensic Medicine,Wenzhou medical college,Zhejiang Wenzhou,325000,China) |
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| Abstract: | Objective To establish a new method for GC-rs7041 SNP typing using mismatch primer-induced restriction sites in amplified DNA and study the application.Methods To type the GC-rs7041 nt301 SNP,the template was amplified with the mismatch primers nt281-nt300.The mismatch primers with the presence of nt301 SNP created a XhoI restriction site.Subsequently,183 templates from a Han population of Wenzhou city were restricted separately using the enzymes XhoI which clearly identified nt301 SNP.Results Typing result... |
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| Keywords: | forensic biology evidence group specific component(GC) mismatch primer-induced RFLP genetic polymorphism SNP |
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