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Constructing STR Multiplexes for Individual Identification of Hungarian Red Deer |
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| Authors: | Zoltan Szabolcsi MSc Balazs Egyed PhD Petra Zenke PhD Zsolt Padar PhD Adrienn Borsy PhD Viktor Steger PhD Erzsebet Pasztor PhD Sandor Csanyi PhD DSc Zsuzsanna Buzas PhD Laszlo Orosz PhD DSc |
| Institution: | 1. Department of Genetics, E?tv?s Loránd University, Pázmány Péter s. 1/c, 1117 Budapest, Hungary;2. Institute of Molecular Genetics, Agricultural Biotechnology Center, Szent‐Gy?rgyi Albert u. 4, 2100 G?d?ll?, Hungary;3. Department of Genetics, Institute for Forensic Sciences, Mosonyi u. 9, 1087 Budapest, Hungary;4. Genoid Ltd., Szanatórium u. 19, 1528 Budapest, Hungary;5. Department of Animal Breeding, Nutrition and Laboratory Animal Science, Faculty of Veterinary Science, Szent István University, István u. 2, 1078 Budapest, Hungary;6. Institute of Molecular Pharmacology, Research Centre for Natural Sciences, Pusztaszeri u. 59‐67, 1025 Budapest, Hungary;7. Institute for Wildlife Conservation, Szent István University, Páter Károly u. 1, 2100 G?d?ll?, Hungary;8. Directorate General of National Institute of Pharmacy, Zrínyi u. 3, 1051 Budapest, Hungary;9. Additional information and reprint requests:;10. Laszlo Orosz, Ph.D., D.Sc.;11. Department of Genetics;12. E?tv?s Loránd University;13. Pázmány Péter s. 1/c;14. 1117 Budapest;15. Hungary;16. E‐mail: |
| Abstract: | Red deer is the most valuable game of the fauna in Hungary, and there is a strong need for genetic identification of individuals. For this purpose, 10 tetranucleotide STR markers were developed and amplified in two 5‐plex systems. The study presented here includes the flanking region sequence analysis and the allele nomenclature of the 10 loci as well as the PCR optimization of the DeerPlex I and II. LD pairwise tests and cross‐species similarity analyses showed the 10 loci to be independently inherited. Considerable levels of genetic differences between two subpopulations were recorded, and FST was 0.034 using AMOVA. The average probability of identity (PIave) was at the value of 2.6736 × 10?15. This low value for PIave nearly eliminates false identification. An illegal hunting case solved by DeerPlex is described herein. The calculated likelihood ratio (LR) illustrates the potential of the 10 red deer microsatellite markers for forensic investigations. |
| Keywords: | forensic science wildlife forensic genetics Cervus elaphus hippelaphus red deer tetranucleotide microsatellite deer genetic identification population structure |