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Forensic Informativity of ~3000 bp of Coding Sequence of Domestic Dog mtDNA |
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| Authors: | Helen Angleby BEng Mattias Oskarsson PhD Junfeng Pang PhD Ya‐ping Zhang PhD Thomas Leitner PhD Caitlyn Braham BS Lars Arvestad PhD Joakim Lundeberg PhD Kristen M Webb PhD Peter Savolainen PhD |
| Institution: | 1. Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, School of Biotechnology, , SE‐171 65 Solna, Sweden;2. State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, , Kunming, 650223 Yunnan, China;3. Los Alamos National Laboratory, Theoretical Biology and Biophysics, MS K710, , Los Alamos, NM, 87545;4. Department of Biology, Allegheny College, , Meadville, PA, 16335;5. Department of Numerical Analysis and Computer Science, Swedish e‐Science Research Centre, Stockholm University, , SE‐171 21 Stockholm, Sweden;6. Science for Life Laboratory, Department of Computational Biology, KTH Royal Institute of Technology, School of Computer Science and Communication, , SE‐171 65 Solna, Sweden |
| Abstract: | The discriminatory power of the noncoding control region (CR) of domestic dog mitochondrial DNA alone is relatively low. The extent to which the discriminatory power could be increased by analyzing additional highly variable coding regions of the mitochondrial genome (mtGenome) was therefore investigated. Genetic variability across the mtGenome was evaluated by phylogenetic analysis, and the three most variable ~1 kb coding regions identified. We then sampled 100 Swedish dogs to represent breeds in accordance with their frequency in the Swedish population. A previously published dataset of 59 dog mtGenomes collected in the United States was also analyzed. Inclusion of the three coding regions increased the exclusion capacity considerably for the Swedish sample, from 0.920 for the CR alone to 0.964 for all four regions. The number of mtDNA types among all 159 dogs increased from 41 to 72, the four most frequent CR haplotypes being resolved into 22 different haplotypes. |
| Keywords: | forensic science domestic dog mitochondrial DNA coding region control region exclusion capacity |