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The Optimal Combination of Cartilage Source and Apparatus for Long‐Term In Vitro Chondrocyte Viability Analysis |
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| Authors: | Armin Alibegović MD PhD Jože Balažic MD PhD Danijel Petrovič MD PhD Nevenka Kregar Velikonja PhD Rok Blagus PhD Dušan Šuput MD PhD Matej Drobnič MD PhD |
| Institution: | 1. Institute of Forensic Medicine, Medical Faculty, University of Ljubljana Korytkova 2, 1000 Ljubljana, Slovenia.;2. Institute of Histology and Embryology, Medical Faculty, University of Ljubljana, Korytkova 2, 1000 Ljubljana, Slovenia.;3. Educell d.o.o., Letali?ka 33, 1000 Ljubljana, Slovenia.;4. Institute of Biostatistics and Medical Informatics, Medical Faculty, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia.;5. Institute of Pathophysiology, Medical Faculty, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia.;6. Department of Orthopedic Surgery, University Medical Centre Ljubljana, Zalo?ka 9, 1000 Ljubljana, Slovenia. |
| Abstract: | Abstract: Most studies of long‐term chondrocytes survival were for tissue banks. They showed a gradual reduction in the viable chondrocytes percentage as a function of time and ambient temperature, but the samples were harvested under optimal conditions. The aim of our study was to determine the most reliable combination of cartilage source and assay for the in vitro postmortem chondrocyte viability analysis in the conditions that imitate a dead body. Osteochondral cylinders were procured from femoral condyles and talar trochleas of three male donors and stored in the cell culture media at 4 ± 2°C and 23 ± 2°C. The samples were analyzed by a cell viability analyzer and a confocal laser scanning microscope (CLSM) initially 24–36 h after death and then in 4‐week intervals. The results reconfirmed the significant influence of time (p = 0.0002), but not of the temperature (p = 0.237). The largest reproducibility was presented for the knee joint and the CLSM. |
| Keywords: | forensic science forensic pathology time since death articular cartilage chondrocyte viability temperature confocal laser scanning microscope cell viability analyzer |