纤维蛋白原在心肌梗死死后诊断的特异性研究

为探讨纤维蛋白原(Fg)在心肌梗死死后诊断的特异性,应用免疫组织化学和图像分析技术,对正常心脏、心肌梗死及其它非梗死性的引起直接或间接心脏损害的情况如心肌炎、窒息、电击死、出血性休克、心挫伤、有机磷中毒等心肌细胞内Fg的变化进行研究。结果发现:正常对照组心肌细胞内未见Fg阳性反应,而心肌梗死、心肌炎、窒息、电击、休克、心挫伤、有机磷中毒等组均可见Fg阳性反应,且各组Fg阳性反应面积与正常对照组存在显著性差异。因此Fg作为心肌梗死死后诊断指标,易受心肌炎、窒息、电击、休克、心挫伤、有机磷中毒等的影响,对诊断心肌梗死的特异性较差。

Study on the Specificity of Fibrinogen for the Postmortem Diagnosis of Myocardial Infarction

In order to explore the specificity of fibrinogen(Fg)in the postmortem diagnosis of myocardial infarction,the changes of Fg staining in normal, infarcted and non-infarcted myocardia with direct of indirect myo-cardial injuries(myocarditis,mechanical asphyxia,electrocution,hemorrhagic shock,cardiac contusion and organophosphate poisoning,etc.)were studied with immunohistochemistry and an image andalysis system.The results showed that Fg was negative in the normal myocardia,while positive in the infarcted as well as in the non-infacted with myocardial injuries.The positive staining areas were significantly different from that of the normal control group.lt is concluded that positive reaction of Fg could be easily affected by myocarditis,mechanical asphyxia,electrocution,hemorrhagic shock,cardiaccontusion,and organophosphate poisoning so it is not specific for the diagnosis of myocardial infarction.