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胶体金标记苯丙胺单克隆抗体免疫试剂盒研究
引用本文:曾立波,陈连康,胡小龙,张艳,骆华,张玉荣,陈民,张润生.胶体金标记苯丙胺单克隆抗体免疫试剂盒研究[J].中国司法鉴定,2007(6):31-33.
作者姓名:曾立波  陈连康  胡小龙  张艳  骆华  张玉荣  陈民  张润生
作者单位:上海市公安局物证鉴定中心,上海,200083
摘    要:目的建立一种准确、快速、简便的检测尿液中苯丙胺(AMP)的胶体金免疫层析技术。方法采用柠檬酸三钠还原法制备胶体金颗粒,标记抗AMP单抗,将AMP—BSA抗原固相于硝酸纤维素膜上,制备胶体金免疫层析测试条。通过尿液、血液和唾液中的可能存在的苯丙胺成分与测试条上的苯丙胺-BSA完全抗原竞争结合有限的单抗结合位点,来判定检测结果。结果用ICT法和GC/MS检测217份尿样,本法检测阈值为1000ng/mL,特异性为99.17%,准确性为99.54%。结论ICT法检测尿液中的AMP特异性强,灵敏度高、简便快速、无需特殊仪器设备,具有广泛应用价值。

关 键 词:胶体金  单克隆抗体  苯丙胺  试剂盒
文章编号:1671-2072-(2007)06-0031-03
修稿时间:2007年8月27日

Study on the Immunochromatographic Test for the Detection of Amphetamine
ZENG Li-bo,CHEN Lian-kang,HU Xiao-long,ZHANG Yan,LUO Hua,ZHANG Yu-rong,CHEN Min,ZHANG Run-sheng.Study on the Immunochromatographic Test for the Detection of Amphetamine[J].Chinese Journal of Forensic Sciences,2007(6):31-33.
Authors:ZENG Li-bo  CHEN Lian-kang  HU Xiao-long  ZHANG Yan  LUO Hua  ZHANG Yu-rong  CHEN Min  ZHANG Run-sheng
Abstract:Objective To develop an accurate,rapid and simple colloidal gold immuno-chromatographic test(ICT) for the detection of amphetamine in urine.Methods Colloidal gold was prepared by reduction of HAuCl4 with trisodium citric acid and conjugated with anti-amphetamine monoclonal antibody.Amphetamine-BSA conjugate was made and coated on nitrocellulose membrane to get the test.Outcome was detectable as a result of the competition for limited antibody binding sites between amphetamine-BSA and free amphetamine which may be present in urine.Results A total of 217 urine samples were tested for amphetamine by ICT and GC/MS.The cut-off value was 1000ng/mL,specificity was 99.17% respectively and the accuracy was 99.54%.Conclusion ICT is a sensitive,rapid and simple assay for detecting amphetamine in urine.
Keywords:colloidal gold  monoclonal antibody  amphetamine
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