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| 甲醛溶液固定对ICP-MS检测电流损伤皮肤金属化的影响 | |
| 引用本文： | 张建华,陈圆圆,马栋,李立,陈忆九.甲醛溶液固定对ICP-MS检测电流损伤皮肤金属化的影响[J].中国司法鉴定,2011(1):26-29. |
| 作者姓名： | 张建华 陈圆圆 马栋 李立 陈忆九 |
| 作者单位： | 1. 司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海,200063 2. 司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海200063;苏州大学医学部法医学系,江苏苏州215123 3. 司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海200063;复旦大学上海医学院法医学系,上海200032 |
| 基金项目： | 中央级科研院所公益性项目 |
| 摘 要： | 目的探讨应用电感耦合等离子体质谱（inductively coupled plasma-mass spectrometry ICP-MS）技术检测甲醛溶液固定后电流损伤皮肤中金属元素。方法利用黄铜、铁电极材料以220V交流电电击兔子后肢,电损伤皮肤分别进行甲醛溶液固定1周、6个月后,应用ICP-MS对电流损伤皮肤中金属元素进行测定。结果与对照组皮肤比较,黄铜电击组皮肤中检测出Cr、Ni、Cu、Zn、Pb含量升高（P〈0.05）;固定1周、6个月后仍可检测出Cr、Ni、Cu、Zn、Pb成分,其中Cr、Cu、Zn、Pb含量电击组与固定组差异无统计学意义。与对照组比较,铁电击后皮肤中检测出Fe、Cr、Mn、Ni元素含量升高（P〈0.05）;固定组（1周和6个月）后Fe、Cr、Ni元素含量与电击组差异无统计学意义。结论 ICP-MS可作为检测电流损伤皮肤中金属元素的有效方法,甲醛溶液固定后触电皮肤仍可进行沉积的金属元素检测,且固定对皮肤金属元素的含量影响较小,该方法仍可为电流损伤诊断提供依据。 |
| 关 键 词： | 法医病理学 电击伤 皮肤 电感耦合等离子体质谱仪 甲醛溶液 金属化 兔 |
Influence of Formalin Fixation on ICP-MS Detection of Metal Elements in Skin in Electric Injury |
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| ZHANG Jian-hua,CHEN Yuan-yuan,MA Dong,LI Li,CHEN Yi-jiu.Influence of Formalin Fixation on ICP-MS Detection of Metal Elements in Skin in Electric Injury[J].Chinese Journal of Forensic Sciences,2011(1):26-29. | |
| Authors: | ZHANG Jian-hua CHEN Yuan-yuan MA Dong LI Li CHEN Yi-jiu |
| Institution: | ZHANG Jian-hua1,CHEN Yuan-yuan1,2,MA Dong2,LI Li1,3,CHEN Yi-jiu1(1.Shanghai Key Laboratory of Forensic Medicine,Institute of Forensic Science,Ministry of Justice,Shanghai 200063,China,2.Department of Forensic Medicine,Medical College of Soochow University,Suzhou 215123,3.Department of Forensic Medicine,Shanghai Medical College,Fudan University,Shanghai 200032,China) |
| Abstract: | Objective To detect metal elements in skin in electric injury by inductively coupled plasma mass spectrometry(ICP-MS) after fixed by formalin.Methods Brass and iron were used as electrodes to make electrical marks on rabbit hind limbs(220v AC),and the injured skin was fixed in formalin for 1 week or 6 months separately.ICP-MS was applied to detect metal elements in the injured skin.Results Compared with the control group,the levels of chromium,nickel,copper,zinc and lead were higher in the group of brass sh... |
| Keywords: | forensic pathology electric injury skin inductively coupled plasma mass spectrometry(ICP-MS) formalin metallization rabbit |
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