| 发光细菌在法医毒物检测中的应用 |
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| 引用本文： | 曾立波,陈连康,杨键,夏桂珍,丁国荣,张玉荣,张润生,王菁,曹芳琦.发光细菌在法医毒物检测中的应用[J].中国司法鉴定,2014(3):33-35. |
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| 作者姓名： | 曾立波 陈连康 杨键 夏桂珍 丁国荣 张玉荣 张润生 王菁 曹芳琦 |
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| 作者单位： | 1. 上海市刑事科学技术研究所,上海,200083
2. 武警政治学院,上海,200435 |
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| 基金项目： | 上海市科学技术委员会资助项目 |
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| 摘 要： | 目的利用发光细菌-青海弧菌Q67对于法医毒物的敏感性,建立一种案发现场快速检测法医毒物的发光细菌检测方法。方法制备冷冻干燥青海弧菌Q67测试液,将法医毒物检材的不同浓度稀释液加入制备好的青海弧菌Q67测试液中,通过检测青海弧菌Q67反应前后的相对发光强度的变化,计算出样本对青海弧菌Q67相对发光强度的变化率或抑制率,对法医毒物检材的毒性进行生物影响的定性评价。结果通过对包含多种农药混合物的法医检材稀释液的测试,发现发光细菌检测结果呈阳性的4个检材对青海弧菌Q67的发光强度抑制率分别为：100.00%,90.41%,84.26%,88.81%。结论通过对包含多种农药混合物的法医检材的测试表明,青海弧菌Q67发光检测方法对法医毒物的毒性的检测是一种快速、灵敏的现场检测方法。
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| 关 键 词： | 法医毒物分析 发光细菌 毒物检测 毒性评价 |
Evaluation of Toxicity by the Luminescent Bacteria |
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| Institution: | ZENG Li-bo, CHEN Lian-kang, YANG Jian, XIA Gui-zhen, DING Guo-ron, ZHANG Yu-rong, ZHANG Run-sheng, WANG Jing, CAO Fang-qi, LU Xin-wei (1. Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China; 2. Political College of Military Police, Shanghai 200435, China) |
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| Abstract: | Objective To establish a rapid method for the onsite evaluation of forensic samples＇ toxicity by the luminescent bacteria V.qinghaiensis Q67, which are toxicity sensitive. Method A series of diluted forensic samples which contained several kinds of pesticides were added to lyophilized V.qinghaiensis Q67. The toxicity can be determined by the inhibition ratio of the bacteria luminescent intensity. Results The luminescent intensity inhibition ratios of four positive samples were 100.00%, 90.41%, 84.26% and 88.81% respectively. Conclusion The luminescent intensity assay for V.qinghaiensis Q67 is a rapid and sensitive onsite method for evaluating the toxicity of forensic samples. |
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| Keywords: | forensic toxicology luminescent bacteria toxicity assay toxicity evaluation |
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