唾液中乙醇含量检测试剂条的研究

目的根据唾液和血液中乙醇含量相关性的实验结果,建立了一种简便快速、准确可靠的检测唾液中乙醇含量的方法。方法本方法利用酶学原理,将一定量乙醇氧化酶(ALO)和过氧化物酶以及底物四甲基联苯胺(TMB)固定于试剂条上,当样本中含有乙醇时,酶学反应使底物TMB显色,通过比对反应的不同颜色,对样本中乙醇质量浓度进行半定量。结果用本方法检测300个自愿者的唾液,和用GC/MS法对照检测志愿者唾液中的乙醇含量,定量结果基本一致。本产品检测过程仅需2min,其检测的阈值为0.1mg/mL,敏感度为96.5%,特异性为91%,准确性为94.7%。结论采用酶学方法制备的乙醇含量检测试剂条,通过显色反应对唾液中的乙醇含量进行半定量检测,其特点为快速简便、准确可靠,适合现场使用。

Study of Rapid Test Strip for Detecting Alcohol in Saliva

Objective To develope a reliable rapid test strip for detecting alcohol in saliva according to the relativity of the alcohol concentration in saliva and blood. Methods The alcohol oxidase (ALO) in the presence of peroxidase and its substrate TMB was coated on the test strip by using enzymatic technology. The distinct color could be observed when alcohol exists in the saliva sample. The alcohol concentration could be semi-quantified by comparing color developed on the test strip. Result The alcohol concentration in saliva specimens of 300 volunteers were tested by the alcohol rapid test strip and GC/MS, respectively. The results detected by the two methods were almost the same. The cut-off value of alcohol rapid test strip was 0.1mg/mL, sensitivity 96.5%, specificity 91%, and accuracy 94.7%. Conclusion Alcohol rapid test strip with enzymatic technology is sensitive,rapid and easy to handle in detecting alcohol in saliva, and is suitable for onsite testing.