卡西酮、甲卡西酮和4-甲基甲卡西酮的 LC-MS/MS分析方法的研究

目的 建立卡西酮、甲卡西酮、4-甲基甲卡西酮的LC-MS/MS定性定量分析方法.方法 采用Agilent 6460三重串联四极杆液质联用仪（LC/QQQ）,样品用甲醇直接提取,采用Agilent Zorbax（R）Eclipse Plus C18色谱柱（100mm×2.1mm,1.8μm）,流动相为0.1％甲酸和乙腈,梯度洗脱,流速为0.3mL/min,进样体积为3μL.质谱应用ESI源、正离子模式、多反应监测（MRM）方式检测卡西酮、甲卡西酮、4-甲基甲卡西酮.结果 卡西酮的线性范围为1ng/mL～25000ng/mL,甲卡西酮的线性范围为0.1ng/mL～～10000ng/mL,4-甲基甲卡西酮的线性范围为1ng/mL～10000ng/mL.结论 该方法简单、准确,灵敏度高,可以满足案件鉴定工作的需要.

Qualitative and quantitative analysis of cathinone,methcathinone and mephedrone by LC-MS/MS

Objective To establish qualitative and quantitative analysis methods of cathinone, methcathinone and mephed tone by LC-MS/MS. Methods Agilent 6460 LC/QQQ was used, samples were extracted with methanol and separated with Agi lent Zorbax Eclipse Plus C18 column（100mm×2.1mm,1.8μm）, mobile phase was 0. 1 % FA and Acetonitrile, Gradient elution, the flow rate was 0. 3mL/min, and the injection volume was 3μp L. The concentration of cathinone, methcathinone and mephed- rone were detected with multiple reactions monitoring （MRM） mode. Results Cathinone＇s linear range was 1-25000ng/mI., Methcathinone＇s linear range was 0. 1-10000 ng/mL, and Mephedrone＇s linear range was 1-10000ng/mL. Conclusion This meth- od is accurate and has high sensitivity for quantitative detection of cathinone, methcathinone and mephedrone.