液相色谱-质谱联用同时检测17种常见毒品的定性分析方法

目的 建立同时检测17种常见毒品的液相色谱-质谱联用定性分析方法.方法 对海洛因、吗啡、冰毒、氯胺酮等17种毒品的色谱行为进行了研究,对流动相梯度程序和质谱参数进行了细致考察和优化.采用岛津Shim-pack XR-ODS（100mm×2.0mm,2.2μm）色谱柱,流动相A：0.3％甲酸;流动相B：0.3甲酸乙腈,梯度洗脱程序0min～3min～8min～15min～20min,3％B～18％B～23％B～93％B～93％B,雾化电压5kV,扫描速度3750u/sec,雾化气流量1.5L/min,干燥气流量10L/min.结果 所测17种毒品的21种组分均获得了良好的分离效果和质谱响应,scan模式下检测限（S/N≥3）1.9pg/μL～40.0pg/μL.结论 该方法快速、简便、高效,适合公安办案中对未知毒品进行定性检测.

Simultaneous detection of 17 kinds of abused drugs by LC-MS

Objective To establish a method for simultaneous detection of 17 kinds of abused drugs. Methods The separation was performed on a Shim-pack XR-ODS column （100min× 2. 0mm, 2. 2μm）, using acetonitrile（B） water（A） with 0. 3% formic acid as mobile phase. The gradient elution procedure was 0min～3min～ 8min～ 15min～ 20min, 3% B～ 18%B～ 23%B～ 93%B～93%B. The interface voltage, scan speed, nebulizing gas flow, drying gas flow was set at 5kV, 3750u/see, 1.5L/rain, 10L/rain respectively. Results 21 compounds in 17 kinds of abused drugs gained good seperative efficiency and mass respond. The limits of detection （S/N≥3） were 1.9pg/μL-40.0pg/μL under scan mode. Conclusion This method was rapid, simple, effective and suited for simultaneous detection of unkown drug samples.