浙江宣城地区汉族人群18个STR基因座遗传多态性

目的 调查浙江宣城地区汉族人群5000例无关个体18个STR基因座多态性分布.方法 应用AmpFlSTR Identifiler荧光标记复合扩增系统检测,统计计算群体遗传学参数.结果 18个STR基因座的基因型分布均符合Hardy-Weinberg平衡（P＞0.05）,共检出270个等位基因,频率在0.0001～0.5260之间,共有1289种基因型,在13个基因座上检出共44个微变异等位基因.18个基因座的杂合度观察值（Ho）在0.6238～0.9120之间,杂合度理论值（He）在0.6961～0.9601之间,多态信息含量（PIC）在0.5578～0.9126之间,累计个人识别率（TDP）为0.999 999 999 999 999 999 999 953,三联体累计非父排除概率（CPE）为0.999 999 993487 306,二联体累计非父排除率（CPE＊）为0.985819169.结论 本文调查结果与以往文献报道的不同地区民族的人群等位基因频率资料有一定程度的差异性.上述18个STR基因座适用于宣城地区汉族群体各类案件的法医学个体识别和亲权鉴定.

Genetic polymorphism of 18 STR loci of Han population in Zhejiang Xuancheng area

Objective To investigate genetic polymorphism of 18 STR loci of the Han population in Xuancheng area. Methods Allele frequencies of 18 STR loci for 5000 unrelated Chinese individuals in Xuaneheng area were determined with Amp F1STR Identifiler kit and the values of genetic parameters for Forensic using were calculated. Results No deviations of the ob served allele frequency from Hardy Weinberg equilibrium expectations were found for Cbi-square test （P〉0.05）. 270 alleles and 1289 genotypes were observed. Allele frequencies varied between 0. 0001 and 0. 5260. 44 off ladder alleles （at 13 STR loci） were found. Observed heterozygosity （Ho） varied between 0. 6238 and 0. 9120, theoretical heterozygosity （He） varied between 0. 6961 and 0. 9601,polymorphic information content （PIC） varied between 0. 5578 and 0. 9126, total discrimination power （TDP） of 18 STR loci was 0. 999 999 999 999 999 999 999 953, cumulative probability of paternity exclusion （CPE） for triplet cases was 0. 999 999 993 487 306,CPE for fatherless or motherless cases（CPE ＊ ） was 0. 985819169. Conclusion The results showed the 18 STR loci can be used for forensic personal identification and paternity testing.