| 线粒体DNA短片段复合扩增系统鉴别种属的研究 |
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| 引用本文： | 戈文东,裴黎,倪萍娅,杨雪莹,徐小玉,张颖.线粒体DNA短片段复合扩增系统鉴别种属的研究[J].刑事技术,2011(6):20-21. |
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| 作者姓名： | 戈文东 裴黎 倪萍娅 杨雪莹 徐小玉 张颖 |
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| 作者单位： | 1. 中国人民公安大学,北京100038;公安部法医遗传学重点实验室,北京100038
2. 公安部物证鉴定中心,北京100038;公安部法医遗传学重点实验室,北京100038 |
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| 基金项目： | 中国人民公安大学学生科研创新项目资助 |
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| 摘 要： | 目的建立线粒体DNA短片段复合扩增体系用于种属鉴定的方法。方法提取人、牛、猪、羊、鸡的DNA,用所选的3对引物复合扩增细胞色素b基因（cyt b）片段、16srRNA基因片段和ND4基因片段,扩增产物经琼脂糖凝胶电泳检测。结果人DNA扩增产物在358bp、157bp和110bp处各出现一条带;动物DNA扩增产物均只有358bp一条带。结论线粒体DNA短片段复合扩增鉴别种属的方法可区分人源性生物检材和其它动物样本,可应用于法庭科学实践。
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| 关 键 词： | 线粒体DNA cytb基因 16srRNA基因 ND4基因 种属鉴定 |
A study of species identification by multiple amplification of short mitochondrial DNA fragment |
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| GE Wen-dong,PEI Li,NI Ping-ya,et al.A study of species identification by multiple amplification of short mitochondrial DNA fragment[J].Forensic Science and Technology,2011(6):20-21. |
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| Authors: | GE Wen-dong PEI Li NI Ping-ya |
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| Institution: | GE Wen-dong,PEI Li,NI Ping-ya,et al(Chinese People's Public Security University,Beijing 100038,China) |
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| Abstract: | Objective To establish a multiple amplification system of short fragment located in mitochondrial DNA for species identification.Methods Extract DNA from human and other animals including cattle,pig,sheep and chicken.Three pairs of primers were mixed in the same reaction and the PCR amplification products were analyzed by agarose gel electrophoresis.Results Three bands(358bp,157bp,110bp) were obtained from human DNA,while only one band(358bp) obtained from nonhuman DNA.Conclusion The method of species ident... |
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| Keywords: | mtDNA cyt b gene 16srRNA gene DN4 gene species identification |
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