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两种方法提取汗潜手印DNA的比较研究
引用本文:郝金萍,邵丽芳,陈松,刘开会,郭燕霞.两种方法提取汗潜手印DNA的比较研究[J].刑事技术,2013(4):34-35.
作者姓名:郝金萍  邵丽芳  陈松  刘开会  郭燕霞
作者单位:1. 公安部物证鉴定中心,北京,100038
2. 北京市公安局刑侦总队七支队,北京,100054
摘    要:目的比较M48和DNeasy○R plant Mini两种方法提取汗潜手印DNA的优劣。方法用M48和DNeasy○Rplant Mini两种方法分别提取16对汗潜手印DNA,并进行DNA定量,比较定量结果。结果 M48法明显比plant Mini法提取到的DNA量多(配对t检验:α=0.05,t=3.45,γ=15,0.002

关 键 词:法庭科学  汗潜手印  DNA提取

The comparison of two methods for extracting DNA from sweat latent fingerprints
Institution:HAO Jin-ping,SHAO Li-fang,CHEN Song,et al(Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China)
Abstract:Objective To compare QIAGEN MagAttract○ R DNA Mini M48 kit and DNeasy○ R plant Mini kit in DNA extraction from sweat fingerprints.Methods DNA on 16 pairs of fingerprints was extracted using QIAGEN MagAttract○ R DNA Mini M48 kit and DNeasy○ R plant Mini kit respectively,and then the extract was analyzed.Results A significant increase in DNA recovery was noted by using QIAGEN MagAttract○ R DNA Mini M48 kit as compared to DNeasy○ R plant Mini kit(pared t-test:α=0.05,t=3.45,γ=15,0.002p0.005).Conclusion The QIAGEN MagAttract○ R DNA Mini M48 kit was more effective for DNA recovery from sweat latent fingerprints compared with the DNeasy○ R plant Mini kit.
Keywords:forensic science  sweat latent fingerprints  DNA extraction
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