| 激光显微切割技术与低体系扩增方法的法医学应用探索 |
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| 引用本文： | 曾宪海,李斌.激光显微切割技术与低体系扩增方法的法医学应用探索[J].刑事技术,2012(5):3-5. |
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| 作者姓名： | 曾宪海 李斌 |
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| 作者单位： | 福建省公安厅刑事技术总队,福州350003 |
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| 基金项目： | 福建省自然科学基金资助项目（2010J05051） |
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| 摘 要： | 目的建立减少DNA低体积扩增过程中产生气泡的方法。方法采用激光显微切割技术、PALM系统收集目标细胞,并在1μL～1.5μL低体积扩增样本,加入扩增液0.7μL～0.8μL。结果低体积扩增反应中的失败位点比例为2.3%,比常规反应位点时常会产生气泡导致该位点样本扩增失败且达30%以上的比例显著降低。结论低体积扩增方法可以减少或克服扩增过程中气泡的产生,提高扩增成功率。
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| 关 键 词： | 激光显微切割 低体积扩增 PALM系统 气溶胶 比较 |
Application of Laser Capture Microscope and low volume PCR in forensic DNA analysis |
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| ZENG Xian-hai,LI Bin.Application of Laser Capture Microscope and low volume PCR in forensic DNA analysis[J].Forensic Science and Technology,2012(5):3-5. |
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| Authors: | ZENG Xian-hai LI Bin |
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| Institution: | (Forensic Science Division, Fujian Provincial Public Security Department, Fuzhou 350003, China) |
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| Abstract: | Objective To establish a new method for reducing the bubbles in process of Low Volume PCR （LV-PCR）. Methods Target cells were collected under Laser Capture Microscope and LV-PCR was performed. Results＇ Bubbles can be re- duced by skillful collection operations and LV-PCR amplification. Conclusion The LV-PCR method was better than standard PCR method for analysis of varying concentration DNA samples. |
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| Keywords: | Laser Capture Microscope low volume PCR |
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