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| 可卡因对小鼠离体腹腔巨噬细胞功能的影响 | |
| 引用本文： | 牛勇,孙文平,江南.可卡因对小鼠离体腹腔巨噬细胞功能的影响[J].刑事技术,2012(4):10-12. |
| 作者姓名： | 牛勇 孙文平 江南 |
| 作者单位： | 1. 公安部五局六处,北京,100741 2. 山东省泰山医学院病理生理系,271000 3. 浙江省温州市公安局瓯海区分局,325000 |
| 基金项目： | 国家“十五”重点科技攻关课题 |
| 摘 要： | 目的研究可卡因对体外培养小鼠腹腔巨噬细胞功能的影响。方法无菌分离小鼠腹腔巨噬细胞，制备单细胞悬液，分别用不同浓度的可卡因作用于正常的和LPS活化的腹腔巨噬细胞。MTT法检测其增殖反应；ELISA法检测巨噬细胞培养上清中TNF-α的分泌水平；生物活性检测法检测IL-1的活性。结果可卡因在终浓度10、20和100μtg／mL时能明显抑制LPS刺激的腹腔巨噬细胞增殖反应，减少TNF-α、IL．1的释放，与对照组相比较有显著性差异（P〈0．01）。结论可卡因对体外培养腹腔巨噬细胞功能有明显抑制作用。 |
| 关 键 词： | 法医毒理学 可卡因 巨噬细胞 肿瘤坏死因子-α 白细胞介素-1 |
Effects of cocaine on the function of mouse peritoneal macrophages in vitro |
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| NIU Yong , SUN Wen-ping , JIANG Nan.Effects of cocaine on the function of mouse peritoneal macrophages in vitro[J].Forensic Science and Technology,2012(4):10-12. | |
| Authors: | NIU Yong SUN Wen-ping JIANG Nan |
| Institution: | (Criminal Investigation Department,Ministry of Public Security,Beijing 100038,China) |
| Abstract: | Objective To investigate the effects of Cocaine on the function of mouse peritoneal macrophages in vitro.Methods The single cell suspension of murine peritoneal macrophages was prepared under sterile condition.Different concentrations of Cocaine were added to the normal or LPS-activited peritoneal macrophages.The cell proliferation of macrophages was measured by MTT assay.Concentrations of TNF-α in supernatants were determined by using quantitative sandwich enzymelinked immunosorbent assay(ELISA) kits.The activities of IL-1 were detected by biological assays.Results Compared with those of the normal control groups,the LPS-induced peritoneal macrophages proliferative reactions was inhibited when the cells were exposed to Cocaine ranging from 10μg/mL to 100μg/mL.The productions of TNF-α and IL-1 in supernatants were all reduced markedly(P﹤0.01).Conclusion Cocaine can inhibit the mouse peritoneal macrophages function in vitro. |
| Keywords: | forensic toxicology cocaine macrophages TNF-α IL-1 |
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