| 大鼠脑液压冲击伤后细胞色素C表达的实验研究 |
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| 引用本文： | 陈伟杰,张夔鸣,杜显刚,幸宇,陈诗文,廖志钢.大鼠脑液压冲击伤后细胞色素C表达的实验研究[J].法律与医学杂志,2004,11(3):219-221. |
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| 作者姓名： | 陈伟杰 张夔鸣 杜显刚 幸宇 陈诗文 廖志钢 |
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| 作者单位： | 四川大学华西基础医学与法医学院法医病理教研室 成都610041
(陈伟杰，张夔鸣，杜显刚，幸宇)，福建省宁德市人民检察院 352100
(陈诗文)，四川大学华西基础医学与法医学院法医病理教研室 成都610041(廖志钢) |
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| 摘 要： | 目的 为法医学上闭合性颅脑损伤的早期诊断和损伤时间推断提供依据。 方法 建立大鼠脑液压冲击模型。用免疫组化与图像分析技术分别检测伤后不同时间大鼠脑皮质、海马等部位细胞色素C(CytochromeC ,CytC)的表达。 结果 对照组大鼠脑内CytC有微量表达 ,伤后 15分钟表达开始增强 ,伤后 3小时达高峰 ,1天后开始减弱 ,7天后基本恢复正常。 结论 CytC表达的规律性可望为闭合性颅脑损伤的早期诊断及损伤时间推断提供依据。
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| 关 键 词： | 闭合性颅脑损伤 CytC 免疫组织化学 |
| 文章编号： | 1007-9297(2004)03-0219-03 |
| 修稿时间： | 2004年5月26日 |
A study of the expression of Cyt C after fluid percussion brain injury in rats |
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| CHEN Wei-jie,ZHANG Kui-ming,DU Xian-gang,XING Yu,CHEN Shi-wen,LIAO Zhi-gang. West China School of Basic Medicine and Forensic Sciences of Sichuan University,Chengdu,China, People's Procuratorate of NingDe,Fu Jian ,China.A study of the expression of Cyt C after fluid percussion brain injury in rats[J].The Journal of Law & Medicine,2004,11(3):219-221. |
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| Authors: | CHEN Wei-jie ZHANG Kui-ming DU Xian-gang XING Yu CHEN Shi-wen LIAO Zhi-gang West China School of Basic Medicine and Forensic Sciences of Sichuan University Chengdu China People's Procuratorate of NingDe Fu Jian China |
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| Institution: | CHEN Wei-jie,ZHANG Kui-ming,DU Xian-gang,XING Yu,CHEN Shi-wen,LIAO Zhi-gang. West China School of Basic Medicine and Forensic Sciences of Sichuan University,Chengdu,610041,China, People's Procuratorate of NingDe,Fu Jian 352100,China |
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| Abstract: | Objective To search the objective evidence for the diagnosis on early brain injury and for estimating the time since brain injury. Methods Some rats were hurt by fluid percussion, then sacrifice at different time after injury. The expression of Cyt C was detected by immunohistochemistry, and the results were assessed by image analysis system. Results There was a slight expression of Cyt C in control group. In treat-group, he expression of Cyt C increased in 15 minutes after injury, and it reached at peaks in 3 hours after injury, and it began to decrease in 1 day, but remain above control levels till 7 days. Conclusion The regularity of Cyt C expression might be a useful target for diagnosis of early brain injury, and it also can be used in estimating the time since brain injury. |
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| Keywords: | Closed brain injury Cyt C Lmmunohistochemistry |
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