| ABO基因型检验及其法医学应用研究 |
| |
| 引用本文： | 邱宁,李万水,陈松,张大安.ABO基因型检验及其法医学应用研究[J].法律与医学杂志,1999(1):8. |
| |
| 作者姓名： | 邱宁 李万水 陈松 张大安 |
| |
| 作者单位： | 公安部第二研究所 |
| |
| 摘 要： | 目的采用ＰＣＲ技术对ＡＢＯ血型系统进行基因型检验。方法选择最佳扩增条件进行四引物复合扩增，用限制性内切酶ＫｐｎＩ和ＡｌｕＩ分别酶解扩增产物，电泳分离、银染显色法检验ＡＢＯ基因型。结果对２７０例血斑、２０例混合斑、２０根毛发（有毛囊）、１２份唾液斑等不同的生物检材进行了分型，与血清学方法检验结果相符。结论该方法能够应用于法医学的检验
|
| 关 键 词： | PCR ABO血型系统 基因型 扩增产物限制片段长度多态性（Amp－RFLP） |
The study and application on genotype of the ABO blood groups |
| |
| Qiu Ning,Li Wanshui,Chen Song,et al..The study and application on genotype of the ABO blood groups[J].The Journal of Law & Medicine,1999(1):8. |
| |
| Authors: | Qiu Ning Li Wanshui Chen Song |
| |
| Institution: | Qiu Ning,Li Wanshui,Chen Song,et al. Institute of Forensic Sciences,Ministry of Public Security,Beijing 100038 |
| |
| Abstract: | Objective To detect genotype ABO blood groups using the polymerize chain reaction (PCR). Methods After four primers coamplification, the PCR products were digested by Kpn I and Alu I, followed by 8% polyacrylamide gel to separate and silver staining. Results The genotypes of 270 bloodstains, 20 mix stains, 20 hair roots, 12 saliva stains were compatible with their serotypes. Conclusion The method described here has been applied to forensic casework successfully. |
| |
| Keywords: | PCR ABO Blood group system Genotype Amp RFLP |
| 本文献已被 CNKI 等数据库收录！ |
|
