Rapid determination of carboxyhemoglobin in blood by Oximeter |
| |
Authors: | Brehmer Cornelia Iten Peter X |
| |
Institution: | Institute of Legal Medicine, University of Zurich, Winterthurerstrase 190, CH 8057 Zurich, Switzerland. brec@irm.unizh.ch |
| |
Abstract: | Different methods to determine carboxyhemoglobin (COHb) in blood are described in the literature. In our laboratory three methods to analyze COHb in post-mortem blood samples were compared: the spectrophotometric method of Maehly, a gas chromatographic method with a thermal conductivity detector (GC-TCD) and the Oximeter. Several COHb containing blood samples of deceased persons were analyzed. Results of all three methods were comparable for low concentrations (ca. 10% COHb) as well as for high concentrations (ca. 80% COHb) regardless of the viscosity of the blood samples. The advantages of the Oximeter when compared to Maehly's method and GC-TCD are extreme short time of analysis (<1min), very small blood volume required (<0.1ml) and easy handling. In our opinion application of the Oximeter is not limited to analyses of blood samples from living persons (e.g. in clinical toxicology); it can as well be used for the determination of COHb in post-mortem blood samples. Hence it is a useful and time saving tool in forensic toxicology. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|