Evaluation of five DNA extraction systems for recovery of DNA from bone |
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| Institution: | 1. School of Forensic and Investigative Sciences, University of Central Lancashire, Preston, UK;2. Forensic Division, Department of Chemistry Malaysia (Kimia), Ministry of Science, Technology and Innovation, Malaysia;1. Institute of Forensic Medicine, Faculty of Medicine, University of Ljubljana, Korytkova 2, 1000, Ljubljana, Slovenia;2. Faculty of Tourism Studies - Turistica, University of Primorska, Portoro?, Slovenia;1. Department of Biology, University of Aveiro, Aveiro, Portugal;2. CENCIFOR – Forensic Science Center, Coimbra, Portugal;3. Medicine Faculty, University of Coimbra, Portugal;1. DNA Analysis Laboratory, Natural Sciences Research Institute, University of the Philippines, Diliman, Quezon City, Philippines;2. Program on Forensics and Ethnicity, Philippine Genome Center, National Science Complex, University of the Philippines, Diliman, Quezon City, Philippines;3. Forensic Center, Commission on Human Rights, Central Office, Philippines;4. Department of Anatomy, College of Medicine, University of the Philippines, Manila, Philippines;5. Department of Pathology, College of Medicine, University of the Philippines, Manila, Philippines;6. Department of Anthropology, College of Social Science and Philosophy, University of the Philippines, Diliman, Quezon City, Philippines;1. Institute of Forensic Medicine, Faculty of Medicine, University of Ljubljana, Korytkova 2, 1000 Ljubljana, Slovenia;2. Central Forensic Laboratory of the Police, Research Institute, Aleje Ujazdowskie 7, 00-583 Warsaw, Poland;3. Division of Medical Genetics, Department of Obstetrics and Gynaecology, University Medical Centre Ljubljana, ?lajmerjeva 2, Ljubljana, Slovenia |
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| Abstract: | Five DNA extraction systems were assessed for their DNA extraction efficiency on samples of fresh pig bone. Four commercially available silica-based extraction kits (ChargeSwitch® gDNA Plant Kit (Life Technologies), DNA IQTM System Kit (Promega), DNeasy® Blood & Tissue Kit (Qiagen) and PrepFiler® BTA Forensic DNA Extraction Kit (Life Technologies)) and a conventional phenol-chloroform method were tested in this study. Extracted DNA samples were quantitated with GoTaq® qPCR Master Mix (Promega) using an Applied Biosystems® 7500 Real-Time PCR System and the extracts were amplified using an in-house multiplex system. The phenol-chloroform extraction produced higher yields of DNA than the silica-based extraction methods. Among the silica-based extractions ChargeSwitch® gDNA Plant Kit recovered the highest amounts of DNA. However, all methods produced DNA that could be amplified and none of the extracts contained any detectable inhibition. |
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| Keywords: | DNA extraction Bone Silica-based extraction Forensic |
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