Genetic identification of biological samples buried in clay soil during summer and winter seasons |
| |
| Institution: | 1. Forensic Genetic Service, Centre Branch, National Institute of Legal Medicine and Forensic Sciences, P.I., Coimbra, Portugal;2. CENCIFOR, Forensic Sciences Centre, Portugal;3. Department of Biology, University of Aveiro, Portugal;4. Faculty of Biomedical Sciences “Abel Salazar”, Porto, Portugal;5. Centre Branch of National Institute of Legal Medicine and Forensic Sciences, P.I., Coimbra, Portugal;6. Medicine Faculty of the University of Coimbra, Portugal;1. Laboratorio de Genética Molecular de Cruz Vital – Cruz Roja Ecuatoriana Quito, Ecuador;2. Laboratorio GENES Ltda, Medellin, Colombia;1. Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany;2. IPATIMUP – Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal;3. Forensic Genetics Unit, Institute of Legal Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain;1. IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Portugal;2. Faculty of Sciences of the University of Porto (FCUP), Portugal;3. Center of Biological and Natural Sciences, Federal University of Acre (UFAC), Rio Branco, AC, Brazil;4. DNA Diagnostic Laboratory (LDD), State University of Rio de Janeiro (UERJ), RJ, Brazil;1. Department of Laboratory Science and Technology – Faculty of Health Science – American University of Science and Technology, Lebanon;2. School of Criminal Justice – Faculty of Law and Criminal Justice – University of Lausanne, Switzerland |
| |
| Abstract: | The analysis of a biological samples found in crime scenes can be a challenging task. Minute amount of DNA, degraded DNA and the presence of inhibitors in biological samples, can interfere with the achievement of a complete genetic profile.Chelating resin, silica membranes and silica-coated with paramagnetic resin were the techniques used in this study for the isolation and purification of DNA. Our aim was to find out the best DNA extraction method for application in the presence of biological samples buried in clay soil. |
| |
| Keywords: | DNA extraction Degraded DNA |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
