血细胞线粒体DNA4977缺失与年龄的相关性

目的检测不同年龄组人活体血细胞线粒体DNA4977碱基缺失情况及其与年龄的相关性。方法根据Anderson标准序列设计mtDNA恒定区和mtDNA4977特异缺失区引物,采用实时荧光定量PCR技术,对110份不同年龄组人活体血细胞mtDNA4977缺失水平进行检测。结果在23岁以下个体中未检出mtDNA4977缺失,在大于23岁的被检个体中检测到mtDNA4977缺失,年龄越大,越容易检测到缺失。结论人mtDNA4977缺失与年龄有一定的相关性。

Relationship between human age and mtDNA 4977 deletions in human blood cells

OBJECTIVE: To study the relationship between human age and mtDNA 4977 deletions in human blood cells. METHODS: According to Anderson's sequence, two sets of primers were designed. Human mtDNA 4977 deletions in blood samples from 110 healthy human individuals in different age groups were examined by using Real time quantitative PCR. RESULTS: The mtDNA 4977 deletions could be observed unless the age was under 23 years old. The older the people were, the easier the deletion could be observed. CONCLUSION: mtDNA 4977 deletions in human blood cells were correlated with human ages in some extent.