| 广西苗族人群15个STR基因座的多态性调查 |
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| 作者姓名： | Liu C Yang D Liu CH |
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| 作者单位： | 1. 广州市刑事科学技术研究所,广东,广州,510030
2. 广西壮族自治区公安厅刑科所,广西,南宁,530012 |
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| 基金项目： | 公安部科技攻关项目(20004421401) |
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| 摘 要： | 目的调查广西苗族人群无关个体的15个STR基因座(D8S1179、D21S11、D7S820、CSF1PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、vWA、TPOX、D18S51、D5S818、FGA)多态性,研究其在法医学检验中的应用价值。方法应用AmpFlSTRIdentifilerTM荧光标记复合扩增系统对274例广西苗族无关个体血样DNA进行15个STR基因座的复合扩增,用ABI3100遗传分析仪对扩增产物进行检测,用GeneScan、GenoTyper软件进行基因分型,统计计算15个STR基因座的群体遗传学参数。结果IdentifilerTM荧光标记系统的15个STR基因座在广西苗族人群的累积偶合率为5.04×10-17,累积非父排除率分别为0.9999993。结论该15个STR基因座可满足广西苗族人群法医学的个体识别及亲权鉴定的需要。
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| 关 键 词： | Identifiler~(TM) 复合扩增 荧光标记 STR基因座 遗传多态性 法医学应用 |
| 文章编号： | 1004-5619(2003)04-0204-03 |
| 修稿时间： | 2003年4月22日 |
Genetic studies of 15 STR loci in Guangxi Miao population |
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| Liu C,Yang D,Liu CH.Genetic studies of 15 STR loci in Guangxi Miao population[J].Journal of Forensic Medicine,2003,19(4):204-206. |
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| Authors: | Liu Chao Yang Dian Liu Chang-hui |
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| Institution: | Guangzhou Criminal Science & Technology Institute, Guangzhou 510030, China. |
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| Abstract: | OBJECTIVE: The study was carried out to investigate genetic polymorphism of 15 STR loci in Guangxi Miao population. METHORDS: DNA samples from southern China 274 Miao unrelated individuals were screened by using AmpFlSTR Identifiler PCR Amplification Kit and 3100 Genetic Analyzer. RESULTS: These 15 loci meet the Hardy-Weinberg expectations. The matching probability of the 15 STR loci was 5.04 x 10(-17), and combined paternity of exclution was 0.9999993 in Guangxi Miao population. CONCLUSION: Our results showed Identifiler PCR Amplification systems of 15 STR loci were useful enough to forensic case work in Guangxi Miao population. |
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| Keywords: | Identifiler~(TM) multiplexing PCR STR loci genetic polymorphism forensic sciecce |
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