| 直接扩增法用于重大灾难事故中软骨的个体识别 |
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| 引用本文： | 王传海,徐程,李湘秦,吴勇,杜舟.直接扩增法用于重大灾难事故中软骨的个体识别[J].法医学杂志,2017,33(3). |
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| 作者姓名： | 王传海 徐程 李湘秦 吴勇 杜舟 |
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| 作者单位： | 深圳市公安局刑事警察支队刑事技术处,广东 深圳,518040 |
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| 摘 要： | 目的探讨直接扩增法对软骨STR检验的有效性,以提高灾难受害者身源鉴定工作效率。方法采用Power Plex~21试剂盒对88份软骨进行直接扩增法检验,同步进行磁珠法比较分型结果。结果 88份软骨检材中,直接扩增法与磁珠法均成功检出84份检材的STR基因型,两者分型结果完全一致。结论Power Plex~21试剂盒直接扩增法可以应用于软骨STR分型检验,且操作简便、快速,在重大灾难事故身源鉴定中具有很好的应用前景。
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| 关 键 词： | 法医遗传学 软骨 基因分型技术 直接扩增法 个体识别 |
Individual Identification of Cartilage by Direct Amplification in Mass Disasters |
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| WANG Chuan-hai,XU Cheng,LI Xiang-qin,WU Yong,DU Zhou.Individual Identification of Cartilage by Direct Amplification in Mass Disasters[J].Journal of Forensic Medicine,2017,33(3). |
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| Authors: | WANG Chuan-hai XU Cheng LI Xiang-qin WU Yong DU Zhou |
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| Abstract: | Objective T o explore the effectiveness of direct am plification for the ST R analysis of carti-lage, and to accelerate the effectiveness of disaster victim identification. Methods E ighty-eight cartilage sam ples w ere directly am plified by Pow erPlex誖21 kit, and the results of genotyping w ere com pared w ith that obtained by the m agnetic beads m ethod. Results In 88 cartilage sam ples, the ST R genotypes w ere successfully detected from 84 sam ples by direct am plification and m agnetic beads m ethod, and both the results of genotyping by tw o m ethod w ere consistent. Conclusion D irect am plification w ith Pow er-Plex誖21 kit can be used for ST R genotyping of cartilages. T his m ethod is operated easily and prom ptly, w hich has a potential application in the individual identification of m ass disasters. |
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| Keywords: | forensic genetics cartilage genotyping techniques direct am plification individual identification |
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