利用AutoMate Express~(TM)自动化法医DNA提取系统提取骨骼及牙齿DNA

目的探讨建立骨骼及牙齿DNA自动化提取的新方法。方法将33份骨骼及15份牙齿样本分别用冷冻研磨和手工处理两种方法研磨成粉,采用AutoMate ExpressTM自动化法医DNA提取系统提取DNA并定量。结果 AutoMate ExpressTM自动化法医DNA提取系统能够在3h左右完成骨骼、牙齿DNA的提取,两种方法处理的骨骼样本所得DNA质量浓度差异无统计学意义。冷冻研磨处理的骨骼和牙齿样本均获得了较好的STR分型结果,且牙齿样本所得DNA质量浓度高于手工提取所得。结论应用AutoMate ExpressTM自动化法医DNA提取系统是自动化提取骨骼、牙齿DNA的一种新方法,可应用于法医实际案件检验。

DNA Extraction from Bones and Teeth Using AutoMate ExpressTM Forensic DNA Extraction System

Objective To explore a new method in order to extract DNA from bones and teeth automati- cally. Methods Samples of 33 bones and 15 teeth were acquired by freeze-mill method and manual method, respectively. DNA materials were extracted and quantified from the triturated samples by Auto- Mate ExpressTM forensic DNA extraction system. Results DNA extraction from bones and teeth were completed in 3 hours using the AutoMate ExpressTM forensic DNA extraction system. There was no sta- tistical difference between the two methods in the DNA concentration of bones. Both bones and teeth got the good STR typing by freeze-mill method, and the DNA concentration of teeth was higher than those by manual method. Conclusion AutoMate ExpressTM forensic DNA extraction system is a new method to extract DNA from bones and teeth, which can be applied in forensic practice.