| SSCP法mtDNA分型的法医学应用 |
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| 作者姓名： | Ai HW Yu CY Zhao GS Huang DX Ke J Yue X Yang QE |
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| 作者单位： | 华中科技大学同济医学院法医学系,湖北,武汉,430030 |
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| 摘 要： | 目的探讨SSCP法mtDNA分型在法医鉴定实践中的意义。方法PCR扩增mtDNAHV-I和HV-II的序列多态性片段,直接用SSCP技术分型。对70个湖北汉族真三联家系和140例随机个体进行检测,比较家系中mtDNA的单倍型SSCP图谱。统计分析SSCP法用于两个高变区在母系确定、个人识别中的意义和鉴别能力。结果在70个家系中,母亲和孩子的HV-I、HV-IISSCP带型完全相同;家系中父亲与母子的HV-I图谱不同占98.57%;HV-IISSCP图谱不同占97.13%。140例随机个体的HVI、HVII区分别检出21、16种单倍型,GD值分别为0.9556、0.9356。结论mtDNA-SSCP分型在嫌疑人筛查和母系亲缘关系推断中有实际应用价值。
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| 关 键 词： | 线粒体DNA 单链构象多态性 个人识别 母系家系 |
| 文章编号： | 1004-5619(2006)02-0117-03 |
| 修稿时间： | 2005年11月7日 |
The application of PCR-SSCP in forensic mtDNA typing |
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| Ai HW,Yu CY,Zhao GS,Huang DX,Ke J,Yue X,Yang QE.The application of PCR-SSCP in forensic mtDNA typing[J].Journal of Forensic Medicine,2006,22(2):117-119. |
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| Authors: | Ai Hong-Wei Yu Chun-Ying Zhao Gui-Sen Huang Dai-Xin Ke Ji Yue Xia Yang Qing-En |
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| Institution: | Department of Forensic Medicine, Huazhong University of Science and Technology, Wuhan 430030, China. aimingxu03@163.com |
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| Abstract: | Objective To study the application of PCR-SSCP in forensic mtDNA typing. Methods Primers flanking the mtDNA HV-I and HV-II regions were designed. By PCR-SSCP techniques, 70 family trios and 140 unrelated Wuhan Han individuals were investigated and analyzed. Results In 70 family trios,the SSCP profiles in region HV-I and HV-II of children were not same to that of their fathers in 98.57% and 97.13% respectively but were identical with their mothers. In 140 unrelated Wuhan Han individuals, 21 haplotypes were found in HVI, GD=0.9556; 16 haplotypes were found in HVII, GD=0.9356. Conclusion PCR-SSCP technique may be useful in forensic mtDNA typing, especially for screening the suspects. |
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| Keywords: | mtDNA single strand conformation polymorphisms individual identification maternal genealogy |
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