短串联重复分型异常

短串联重复序列(STR)已被广泛应用于法医学的个体识别和亲子鉴定中。大多数情况下,STR的分型均得到了准确、可靠的结果,但是在应用中也发现了一些异常的分型现象。引起这些异常现象原因很多,有DNA的遗传性变异、DNA模板质量不好、分型试剂或方法的不同、PCR反应中非特异性反应以及异常的电泳行为等。这些异常的结果会干扰STR分型结果的解析。

The unusual typing of short tandem repeats

Short tandem repeatsSTRshave been widely used in forensic sciences such as stain analysis and paternity testing.Although most of STR typing could give the reliable and clear results,some unusual typing have been observed in forensic practice.The anomalous typing could result from a lot of causes,including DNA genetic variation,poor quality or quantity of DNA template,different typing system or method,nonspecific reaction in PCR or anomalous electrophoresis migration.The unusual results may disturb the right interpretation of STR typing.