| FN－Ⅲcs在皮肤切创中的表达与损伤时间关系 |
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| 作者姓名： | Ji Y Zhao ZQ Gu YJ |
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| 作者单位： | 复旦大学医学院法医学系,上海,200032 |
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| 基金项目： | 国家自然科学基金,39770816, |
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| 摘 要： | 目的研究纤维连接蛋白可变剪接片段Ⅲcs在不同损伤时间皮肤中的表达,为早期损伤时间的判定指标提供实验依据.方法DIG标记反意RNA探针,以原位杂交的方法检测大鼠和人体皮肤在损伤早期FNⅢcs表达的变化.结果(1)人体及大鼠皮肤损伤后30min皮肤网状层的毛囊、皮脂腺、血管内皮层中表达FNⅢcs的阳性细胞数开始增多,至伤后6h达到高峰,以后又逐渐减少.(2)人体皮肤FNⅢcs表达的部位与大鼠有明显的差异.结论FNⅢcs的原位检测可望成为判断早期损伤时间的有用指标;为FNⅢcs在创伤修复中的合成方式、部位和作用的研究提供了实验基础.
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| 关 键 词： | FNⅢcs片段 原位杂交 损伤早期时间推断 |
| 文章编号： | 1004-5619(2002)01-0004-03 |
| 修稿时间： | 2001年9月5日 |
The expression of FN-IIIcs in the wound healing and its relationship with injury time |
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| Ji Y,Zhao ZQ,Gu YJ.The expression of FN-IIIcs in the wound healing and its relationship with injury time[J].Journal of Forensic Medicine,2002,18(1):4-6. |
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| Authors: | Ji Yan Zhao Zi-qin Gu Yun-ju |
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| Institution: | Department of Forensic Medicine of Fudan University, Shanghai 200032. |
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| Abstract: | OBJECTIVE: To research the expression of alternative splicing segment of fibronectin-IIIcs in incised wound of skin, to offer the experimental data for early injury time judgement. METHODS: Using in situ hybridization with DIG-labeled anti-sense RNA probe, the expression of FN-IIIcs domain was detected in both human and rat skin incised wound. RESULTS: (1) The expression of FN-IIIcs improved from the time of 30 min after injury, reached peak at 6 h and then reduced gradually. (2) The positive expression cells were mainly distributed in hair follicles, sebaceous glands and endothelium, however, in human beings they were basement cells of epidermis. CONCLUSION: The expression of FN-IIIcs domain would be a supposed useful criteria for early injury time judgement. |
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