人体皮肤切创纤维连接蛋白EDA、EDB的表达与损伤时间关系的研究

目的研究纤维连接蛋白(fibronectin,FN)可变剪接片段EDA、EDB在人体皮肤切创的表达情况,并摸索EDA、EDBmRNA在离体皮肤上最长检出时限,以期为早期损伤时间的推断提供实用的参考指标。方法采用DIG标记的反意RNA探针,原位杂交检测人体皮肤在损伤早期(从30min开始)FNEDA、EDBmRNA表达的变化。结果正常人体皮肤无EDA、EDBmRNA表达;在损伤早期(≤3h)FNEDA、EDB的表达随时间的延长而呈现一种不断上升的趋势;EDA、EDBmRNA主要出现在表皮基底层细胞;EDA、EDBmRNA在标本离体后4h就不能检测到。结论FNEDA、EDB可作为早期损伤时间推断的较理想的参考指标,但原位杂交的检测方法不适用于实际应用。

The relationship between expression of FN in human skin injury and early injury time

Objective In order to supply an effective reference of early injury time estimation and explore the time limit of detection of EDA\EDB mRNA in human skin samples,the expression of alternative splic-ing segment of fibronectin-EDA\EDB in incised wound of human skin were studied.Methods Using in situ hybridization with DIG-labeled anti-sense RNA probe,the expression of FN EDA\EDB domain was detected in human skin incised wound at the early stage of injury(from30min to3h).Results The posi-tive expression rates of FN-EDA\EDB immediately after injury and area far away from wound were same as the control group.The expression of FN-EDA\EDB in human skin incised wound showed a gradually increased tendency in early injury time(within3h).The positive expression cells were mainly distributed in basement cells of epidermis and the expression of EDA is much higher than EDB.It's difficult to detect EDA\EDB mRNA when the samples were deposited in air for4hour.Conclusion FN-EDA\EDB may be used as a sensitive mark for the estimation of early injury time.The in-situ hybridization technique is not applicable in the application.