| 改良AS-PCR引物对ABO基因分型的影响 |
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| 作者姓名： | Wang XJ Diao LJ Feng JZ Lu GP |
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| 作者单位： | 1. 潍坊市公安局,山东,潍坊,261061
2. 山东省公安厅,山东,济南,250001 |
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| 摘 要： | 目的通过改进AS-PCR引物,提高ABO基因型分型正确率。方法把引物P13′末端第5位碱基由G改为C,其分型图谱与改进前进行对比分析。结果改进AS-PCR引物后,OO型非特异产物减少,避免了将OO型误判为AO型。结论引物P13′末端第5位碱基由G改为C后,可有效防止OO型被误判为AO型。
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| 关 键 词： | AS-PCR 改良引物 3′末端 碱基 ABO基因型 |
| 文章编号： | 1004-5619(2006)04-0275-02 |
| 修稿时间： | 2006年3月9日 |
Influence of meliorated AS-PCR primer for ABO genotyping |
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| Wang XJ,Diao LJ,Feng JZ,Lu GP.Influence of meliorated AS-PCR primer for ABO genotyping[J].Journal of Forensic Medicine,2006,22(4):275-276. |
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| Authors: | Wang Xin-Jie Diao Li-Jiang Feng Jian-Zhong Lu Gui-Ping |
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| Institution: | Weifang Public Security Bureau, Weifang 261061, China. |
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| Abstract: | OBJECTIVE: To improve the correct rate of ABO genotyping by meliorating AS-PCR primer. METHODS: The primer P1 was changed into primer P1' by substituting the fifth base G for C of 3' end and the ABO genotyping results of primer P1 and P1' was compared and analysed. RESULTS: The non-specific product of OO typing is reducing and the wrong genotyping of OO and AO was avoided by meliorating AS-PCR primer. CONCLUSION: The rates of wrong ABO genotyping results could be effectively reduced by using altering primer P1'. |
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| Keywords: | AS-PCR |
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