| 大鼠皮肤损伤后纤维连接蛋白剪接异型体的表达 |
| |
| 引用本文： | 刘宁国,赵子琴,陈忆九,顾云菊.大鼠皮肤损伤后纤维连接蛋白剪接异型体的表达[J].法医学杂志,2000,16(2):70-71,74. |
| |
| 作者姓名： | 刘宁国 赵子琴 陈忆九 顾云菊 |
| |
| 作者单位： | 1. 上海医科大学,上海,200032
2. 司法部司法鉴定科学技术研究所,上海,200063 |
| |
| 基金项目： | 国家自然科学基金资助项目!(No 39770 816 ) |
| |
| 摘 要： | 运用地高辛标记纤维连接蛋白 (FN)特殊位点的探针 ,通过原位杂交方法检测不同损伤时间大鼠皮肤各型FN剪接异型体的表达情况 ,结果发现 :伤后大鼠皮肤各型FN的表达随时间呈增高趋势 ;伤后 30～ 6 0min表达出正常皮肤中没有的EⅢA、EⅢB片段。FN的这一特性为法医学诊断生前、死后伤和伤后存活时间推断提供了新指标。
|
| 关 键 词： | 纤维连接蛋白 损伤时间推断 地高辛 原位杂交 |
| 文章编号： | 1004-5619(2000)02-0070-02 |
| 修稿时间： | 1999-08-17 |
The expression of splicing fibranectin variants of cutaneous injuries in rats |
| |
| Liu Ningguo,et al.The expression of splicing fibranectin variants of cutaneous injuries in rats[J].Journal of Forensic Medicine,2000,16(2):70-71,74. |
| |
| Authors: | Liu Ningguo |
| |
| Institution: | Liu Ningguo
(Shanghai Medical University,Shanghai 200032, P, R. China) |
| |
| Abstract: | In situ hybridization with Digoxingenin labeled probes were used to build the relationship between incised cutaneous wounding intervals and the expression of splicing fibronectin variants (total, E III A and E III B Variants) in rats. The results showed that all forms of FN variants increased notably with the wounding time going on. E III A and E III B Variants were expressed abundantly 30 and 60 minutes after injury, while few in normal skin. |
| |
| Keywords: | fibronectin estimation of wounding interval digoxingenin in situ hybridization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
