蛋白质降解与死亡时间推断的初步研究

目的观察大鼠肝脏肌动蛋白、微管蛋白在死后不同时间的降解情况,为死亡时间的推断寻找客观依据。方法大鼠麻醉致死后置于21℃温度控制系统模拟死后18d的改变,在不同时间点剪取肝脏组织抽提蛋白质,利用westernblot技术检测肌动蛋白、微管蛋白的降解变化并对产物进行半定量分析。结果大鼠肝脏肌动蛋白在死后8d尚可检测,死亡10d后检测不到;在死后2d,α微管蛋白已检测不到,但可检测到β微管蛋白,死亡4d后,β微管蛋白也检测不到。结论肌动蛋白、微管蛋白的死后降解存在差异,其在肝脏组织保存时间的不同可作为死亡时间推断的参数。

A study on the relationship between the degradation of protein and the postmortem interval

OBJECTIVE: To observe the degradation of actin and tubulin in the liver tissue of rats after death and to find an objective indicator of the postmortem interval (PMI). METHODS: Female rats were killed under anesthesia by ether and incubated at 21 degrees C in a temperature controlled system to simulate postmortem changes for 18 days postmortem. Protein in the hepatic tissue was extracted, actin and tubulin were then examined by western blot. Thereafter, the semi-quantitative analysis of the image of western blot was performed. RESULTS: Actin in the liver tissue of rats could be detected at 8 days postmortem, but could not be examined after 10 days postmortem. beta-tubulin rather than alpha-tubulin could be examined after 2 days postmortem, and beta-tubulin could not be examined at 4 days postmortem. CONCLUSION: There is some difference in the degradation between actin and tubulin, their different preservation period postmortem may be used as a parameter for PMI estimation.