| 激光捕获显微切割技术用于分离混合斑中精子细胞 |
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| 作者姓名： | Liu F Wang J Yu LJ Guo JZ Gao JW Jiao ZP Tang H |
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| 作者单位： | 山西医科大学法医学院;北京市公安局法医检验鉴定中心; |
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| 摘 要： | 目的 评估激光捕获显微切割(laser capture microdissection.LCM)技术在分离混合斑中少量精子细胞的应用价值.方法 配制不同比例的精液-阴道上皮细胞混合液.分别用差异裂解法和LCM法分离精子细胞,用磁珠法提取精子细胞DNA,并用IdentifilerTM试剂盒进行STR基因型检测.结果 LC...
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| 关 键 词： | 法医遗传学 精子细胞 混合斑 激光捕获显微切割技术 |
Isolating sperm cells by laser capture microdissection technique from mixture sample |
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| Liu F,Wang J,Yu LJ,Guo JZ,Gao JW,Jiao ZP,Tang H.Isolating sperm cells by laser capture microdissection technique from mixture sample[J].Journal of Forensic Medicine,2011,27(1):33-5, 42. |
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| Authors: | Liu Fang Wang Jing Yu Li-juan Guo Jian-zhang Gao Jun-wei Jiao Zhang-ping Tang Hui |
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| Institution: | LIU Fang1,WANG Jing2,YU Li-juan1,GUO Jian-zhang1,GAO Jun-wei2,JIAO Zhang-ping2,TANG Hui2,LIU Ya-cheng2(1.School of Forensic Medicine,Shanxi Medical University,Taiyuan 030001,China,2.Forensic Medical Examination Center,Beijing Public Security Bureau,Beijing 100192,China) |
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| Abstract: | Objective To assess the application value of laser capture microdissection(LCM) technique for isolating a small number of sperm cells from mixture sample.Methods Mixture samples were prepared with sperm cells and vaginal epithelia at different concentrations.Both LCM technique and the differential lysis method were employed to obtain sperm cells from the mixture samples,and DNA was extracted by magnetic beads method.STR genotyping was determined using IdentifilerTM kit.Results The successful STR genotype ra... |
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| Keywords: | forensic genetics sperm cell mixed stain laser capture microdissection(LCM) |
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