| LC-MS/MS-MRM筛选血液中22种有毒生物碱成分 |
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| 作者姓名： | Liu W Shen M Shen BH Xiang P Wu HJ |
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| 作者单位： | 1. 复旦大学上海医学院法医学系,上海,200032;司法部司法鉴定科学技术研究所,上海,200063
2. 司法部司法鉴定科学技术研究所,上海,200063 |
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| 摘 要： | 目的建立液相色谱-串联质谱法对血液中的22种常见有毒生物碱成分进行筛选分析。方法血液以丁丙诺菲为内标经液液提取后,用液相色谱-串联质谱仪以电喷雾电离(ESI )、多反应监测(MRM)方式进行分析。结果以化合物的保留时间、两对母离子/子离子对定性,最低检出限为0.1～20ng/mL。结论该方法选择性佳、灵敏度高,适用于法医毒物分析和临床毒物分析中有毒生物碱的分析。
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| 关 键 词： | 有毒生物碱 筛选 液相色谱-串联质谱法 多反应检测 |
Simultaneous screening for 22 poisonous alkaloids in blood by liquid chromatography-tandem mass spectrometry with multiple-reaction monitoring |
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| Liu W,Shen M,Shen BH,Xiang P,Wu HJ.Simultaneous screening for 22 poisonous alkaloids in blood by liquid chromatography-tandem mass spectrometry with multiple-reaction monitoring[J].Journal of Forensic Medicine,2007,23(5):349-352. |
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| Authors: | Liu Wei Shen Min Shen Bao-hua Xiang Ping Wu He-jian |
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| Institution: | Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China. |
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| Abstract: | OBJECTIVE: To establish a liquid chromatography-tandem mass chromatography (LC-MS/MS) method for the simultaneous screening for 22 poisonous alkaloids in blood. METHODS: This method involves a liquid-liquid extraction (LLE) followed by liquid chromatography-tandem mass spectrometry with multi-ple-reaction monitoring (MRM). After blood was extracted with buprenorphine as the internal standard, the target compounds were analyzed with LC-MS/MS-ESI in the positive ionization mode. RESULTS: Identification was based on the compound's retention time and two precursor-to-product ion transitions. The limits of detection ranged from 0.1 ng/mL to 20 ng/mL in blood. CONCLUSION: The method was sufficiently selective and sensitive to detect poisonous alkaloids and can be applied in forensic and clinical toxicology. |
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| Keywords: | poisonous alkaloids screening LC-MS/MS multiple-reaction monitoring(MRM) |
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