PGM1和EAP同步等电聚焦电泳分型

采用薄层等电聚焦电泳PAGIF，结合反转显色，同时对EAP及PGM1亚型分型。结果在同一块凝胶板以两个表面分别显现出清晰的同工酶谱带，结果互不干扰。EAP检出AA、BB、BA三种表型PGM1检出十种亚型。PAGIF结合双面反转显色可同时对EAP及PGM1亚型分型，累积个体识别率为0．87，可应用于亲子生定。

Simultaneous phenotyping of EAP and PGMI by PAGIF

The study is to find new genetic markers of simultaneousphenotyping and to raise the determinating power ofisoenzymes. Simultaneous electrophoresis of EAP andPGMI by thin-layer PAGIF combined with reversezymogram developing was applied and clear isoenzymebands was observed on both surface of a gel withoutinterference.The method can do typing of the EAP andPGMl at one gel and the accumulative DP is 0.87 It canbe used in paternity test.