| 载体法检验微量检材DNA |
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| 引用本文： | 毛坤云,周建清,顾西蒙,蒋红艳,王禹,陈嘉佳.载体法检验微量检材DNA[J].法医学杂志,2008,24(6):439-441. |
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| 作者姓名： | 毛坤云 周建清 顾西蒙 蒋红艳 王禹 陈嘉佳 |
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| 作者单位： | 镇江市公安局刑事科学技术研究所,江苏镇江,212001 |
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| 基金项目： | 江苏省镇江市科研项目
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| 摘 要： | 目的解决微量生物检材DNA检验难题。方法采用载体法对已知微量血样DNA进行检验，再与目前高灵敏度的Chelex-100提取的DNA检验的相应结果进行比较。结果载体法针对微量血样的DNA检验。不但能得到正确的STR分型结果．同时在检验灵敏度上比Chelex-100法高出2倍以上。结论载体法可提高微量生物栓材中DNA检出率，且操作简单，在法医检案工作中具有较高应用价值。
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| 关 键 词： | 法医遗传学 提取法 载体法 DNA检验 |
Study of DNA Examination in Trace Sample by Carrier Method |
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| MAO Kun-yun,ZHOU Jian-qing,GU Xi-meng,JIANG Hong-yan,WANG Yu,CHEN Jia-jia.Study of DNA Examination in Trace Sample by Carrier Method[J].Journal of Forensic Medicine,2008,24(6):439-441. |
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| Authors: | MAO Kun-yun ZHOU Jian-qing GU Xi-meng JIANG Hong-yan WANG Yu CHEN Jia-jia |
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| Institution: | MAO Kun-yun, ZHOU Jian-qing, GU Xi-meng, JIANG Hong-yan, WANG Yu, CHEN Jia-jia (Institute of Criminal Science, Zhenjiang Public Security Bureau, Zhenjiang 212001, China) |
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| Abstract: | Objective To solve the problems of DNA testing in trace sample. Methods Applying original carrier method to detect known trace blood DNA and to compare it with the results obtained by high effective DNA extracting method of Chelex-100. Results The carrier method not only could obtain the right STR genotype in the trace blood sample, but also was twice as sensitive as the Chelex-100 method. Conclusion The carrier method could improve the DNA detection in trace sample. It is easy to operate and is much more valuable in the forensic case analysis. |
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| Keywords: | forensic genetics extraction carrier method DNA examination |
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