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| TD-RAPD技术用于罂粟品种鉴定初探 | |
| 作者姓名： | Lu F Hong JY He R Li LS |
| 作者单位： | 1. 云南省公安厅刑事科学技术研究所,云南,昆明,650021 2. 昆明明枝科技有限公司,云南,昆明,650223 3. 西双版纳刑事科学技术研究所,云南,景洪,666100 |
| 摘 要： | 目的探索TD-RAPD技术用于罂粟品种鉴定的可行性。方法采用改良CTAB法从罂粟叶片中提取DNA;TD-RAPD技术对种植于云南西双版纳地区的1个罂粟样品进行扩增分析。结果建立了罂粟DNA提取方法,从10个随机引物中筛选出6个引物用于罂粟TD-RAPD分析。结论TD-RAPD技术可用于罂粟DNA的分子标记,为罂粟DNA数据库建立提供技术方法,最终从DNA分子水平上追溯罂粟植物毒源。 |
| 关 键 词： | 罂粟 DNA 降落式随机扩增多态DNA技术 |
| 文章编号： | 1004-5619(2006)05-0367-03 |
| 修稿时间： | 2006年1月5日 |
Study of Papaver somniferum cultivars identification by TD-RAPD technique |
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| Lu F,Hong JY,He R,Li LS.Study of Papaver somniferum cultivars identification by TD-RAPD technique[J].Journal of Forensic Medicine,2006,22(5):367-369. | |
| Authors: | Lu Fan Hong Jun-Yan He Rui Li Li-Sha |
| Institution: | Yunnan Criminal Science and Technology Institute, Kunming 650021, China. |
| Abstract: | Objective The feasibility of Papaver somniferum L. cultivars identification was explored by TD-RAPD technique. Method Genomic DNA was extracted by improved CTAB method. One sample of species from Papaver somniferum L in xishuangbanna area. was studied by using TD-RAPD method. Result We established an optimal method of extracting genomic DNA. Six primers were picked out from 10 primers. Conclusion TD-RAPD could be applied to researches of molecular marker of Papaver somniferum L. TD-RAPD technique provide a method to constitute DNA database of Papaver somniferum L. and conclude the source of opium poppy. |
| Keywords: | Papaver somniferum L DNA TD-RAPD |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |