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| 大鼠死后腓肠肌内糖原含量变化与死亡时间的关系 | |
| 引用本文： | 李斌,陶涛,李倩楠,闫红涛,金菊,黄红,廖志钢.大鼠死后腓肠肌内糖原含量变化与死亡时间的关系[J].法医学杂志,2007,23(3):167-169,F0002. |
| 作者姓名： | 李斌 陶涛 李倩楠 闫红涛 金菊 黄红 廖志钢 |
| 作者单位： | 1. 四川大学华西基础医学与法医学院,四川,成都,610041;四川华大司法鉴定所,四川,成都,610041 2. 四川大学法学院,四川,成都,610041 3. 四川华大司法鉴定所,四川,成都,610041 4. 四川大学华西基础医学与法医学院,四川,成都,610041 |
| 摘 要： | 目的研究大鼠死亡后腓肠肌内糖原含量变化与死亡时间之间的关系。方法采用断颈、机械性窒息、失血性休克三种方法分别处死大鼠,于死亡后0﹑0.5﹑1﹑1.5﹑2﹑3﹑4﹑8﹑12﹑18和24h时间点取腓肠肌,利用PAS染色方法并结合计算机图像分析技术分析糖原含量变化。结果大鼠腓肠肌内糖原含量和阳性面积随死亡后时间的延长而呈线性减少。结论腓肠肌内糖原含量和阳性面积可作为死亡时间推断的参考指标。 |
| 关 键 词： | 腓肠肌 糖原 死亡时间 法医学 |
| 文章编号： | 1004-5619(2007)03-0167-03 |
| 修稿时间： | 2006年12月11 |
A Histochemical Study on the Change of Glycogen Content in Rat Gastrocnemius after Death |
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| LI Bin,TAO Tao,LI Qian-nan,YAN Hong-tao,JIN Ju,HUANG Hon,LIAO Zhi-gang.A Histochemical Study on the Change of Glycogen Content in Rat Gastrocnemius after Death[J].Journal of Forensic Medicine,2007,23(3):167-169,F0002. | |
| Authors: | LI Bin TAO Tao LI Qian-nan YAN Hong-tao JIN Ju HUANG Hon LIAO Zhi-gang |
| Abstract: | Objective To study the relationship between the content of glycogen in gastrocnemius of rat and postmortem interval (PMI). Methods The gastronomies muscles were collected at 0,0.5,1,1.5,2,3,4,6,8,12,18 and 24 hours from rats sacrificed by bleeding, suffocating, and neck breaking, respectively. The changes of glycogen content in these muscles were analyzed by PAS staining and imaging technique. Results The content of glycogen in the rat's gastrocnermius muscle showed a continuous linear decrease in correlation with prolonged postmortem intervals. Conclusion Our data suggest that the changes of glycogen content in rat's gastrocnemius can be used as one of the references for estimation of the postmortem interval. |
| Keywords: | gastrocnemius glycogen postmortem interval forensic medicine |
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