温州汉族人群D12S391、D18S865基因座遗传多态性研究

目的研究短串联重复序列D12S391、D18S865的遗传多态性及法医学应用价值。方法应用聚合酶链反应(PCR)、聚丙烯酰胺凝胶电泳(PAGE)及银染显带技术对温州地区汉族454名无关个体的D12S391、D18S865位点进行分型。结果D12S391观察到11个等位基因,50种基因型;D18S865观察到7个等位基因,21种基因型。基因型频率的分布符合Hardy-Weinberg平衡,两个基因座的观测杂合度(H)分别为0.7974、0.7379;个体识别能力(DP)分别为0.9566、0.9077;多态信息含量(PIC)分别为0.8260、0.7279。结论两个STR基因座具有较高杂合度,等位基因分布符合Hardy-Weinberg平衡(P>0.5),在法医学应用和群体遗传学研究中较高的价值。

Polymorphism of STR loci D12S391/D18S865 in Wenzhou Han population

Objective To investigate the polymorphism and forensic efficiency values of STR loci D12S391?D18S865 Methods Population studies on D12S391 and D18S865 were carried out in a sample of 454 unrelated Wenzhou Han individuals using PCR followed by PAGE and silver stain detection. Results 11?7 alleles and 50?21 genotypes of D12S391?D18S865 were respectively observed among the 454 unrelated individuals. The genotype frequency matched the Hardy- Weinberg equilibrium, The heterozygotes(H) in D12S391?D18S865 were 0.797 4? 0.737 9, Power of Exclusion were 0.956 6? 0.907 7, polymorphism information content(PIC) were 0.826 0? 0.727 9 respectively. Conclusion D12S391 and D18S865 were high polymorphic loci and the frequencies were in accordance with Hardy-Weinberg equilibrium(P>0.5),so the two loci can be used in forensic medicine and in other genetic research areas.