氯胺酮对PC12细胞增殖及凋亡的影响

目的 探讨氯胺酮对肾上腺嗜铬细胞瘤(pheochromocytoma,PC12)细胞的增殖抑制和诱导凋亡的作用及其机制. 方法 以大鼠PC12细胞为多巴胺能神经元的细胞模型,分别以0.9、1.2、1.5、1.8、2.1 mmol/L浓度的氯胺酮加入培养的PC12细胞中,分别培养12、24、48、72h后,用MTT实验检...

Effects of ketamine on proliferation and apoptosis of pheochromocytoma cell

Objective To explore the effect of ketamine on adrenal pheochromocytoma（PC12） cell proliferation inhibition and induction of apoptosis and its mechanism.Methods PC12 cells of rats were models for dopaminergic neuron.PC12 cells were cultured with ketamine at concentrations of 0.9,1.2,1.5,1.8 and 2.1 mmol/L,respectively.The cell viability was measured by MTT method after incubation at 12,24,48 and 72 h.Hoechst stain was used to observe the morphological changes of apoptosis.PC12 cells cultured after 48 h with different concentrations of ketamine were selected to detect apoptotic rate using flow cytometry and detect the expression of bax and bcl-2 proteins using Western blotting.Results For different concentrations of ketamine,vitality of PC12 cells significantly decreased with increase of the incubation time.Apoptosis was obviously observed using Hoechst staining.Flow cytometry showed that apoptosis rates significantly increased with increasing ketamine concentrations.Conclusion Ketamine can inhibit the proliferation of PC12 cell by inducing apoptosis of the PC12 cell in a concentrations-dependent manner.The underlying mechanism may be related to promoting the expression of bax and inhibiting the expression of bcl-2 in the cells.