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| 免疫组织化学法检测HO-1在人挫伤脑组织中的表达 | |
| 作者姓名： | Peng X Jin ZF Shao HJ Gao TL Li Z Zhang YH |
| 作者单位： | 1. 哈尔滨医科大学,法医学教研室,黑龙江,哈尔滨,150081 2. 哈尔滨医科大学,附属第一医院,黑龙江哈尔滨,150081 3. 哈尔滨市公安局,刑事技术支队,黑龙江,哈尔滨,150026 |
| 基金项目： | 黑龙江省政府博士后科研启动基金 |
| 摘 要： | 目的研究人脑挫伤后不同时间血红素加氧酶-1(heme oxygenase-1,HO-1)表达的变化,为法医学脑损伤时间的推断提供依据。方法选择24例因颅脑损伤死亡案例为损伤组,4例非颅脑损伤死亡的案例为对照组,应用免疫组织化学和图像分析技术,分别于脑损伤后存活时间3h、6～9h、12～24h、36h～3d、5～8d及17～20d检测脑组织标本中HO-1随时间的变化规律。结果经统计学分析,HO-1阳性细胞表达在伤后3h以内组开始升高,12～24h组表达强度最高,36h～3d组仍见高表达,以后逐渐下降。各组组间及与对照组相比差异有统计学意义(P<0.05)。结论 HO-1的表达可能成为实际检案中脑损伤时间推断的诊断指标。 |
| 关 键 词： | 法医病理学 脑损伤 血红素加氧酶-1 免疫组织化学 损伤时间 |
Immunohistochemical expression of HO-1 in traumatic human brain tissue |
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| Peng X,Jin ZF,Shao HJ,Gao TL,Li Z,Zhang YH.Immunohistochemical expression of HO-1 in traumatic human brain tissue[J].Journal of Forensic Medicine,2010,26(5):325-7, 331. | |
| Authors: | Peng Xue Jin Zhan-Feng Shao Hong-Jiang Gao Tie-Lei Li Zhong Zhang Ying-Hui |
| Institution: | PENG Xue1,JIN Zhan-feng1,SHAO Hong-jiang1,GAO Tie-lei1,LI Zhong2,ZHANG Ying-hui3(1.Department of Forensic Medicine,Harbin Medical University,Harbin 150081,China,2.The First Affiliated Hospital of Harbin Medical University,3.Criminal Technology Branch,Harbin Public Security Bureau,Harbin 150026,China) |
| Abstract: | Objective To investigate the expression of heme oxygenase-1(HO-1) at different intervals and to provide evidence for estimation on injury intervals after brain contusion in human.Methods Twenty-four patients died of serious brain injury were assigned as injury group and 4 patients died of non-brain injury were served as control group.HO-1 expression was analyzed in brain tissue at different time intervals(3 h,6-9 h,12-24 h,36 h-3 d,5-8 d,17-20 d) by immunohistochemistry and auto-image analysis system.Result... |
| Keywords: | forensic pathology brain injuries heme oxygenase-1 immunohistochemistry injury time |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |