| 大鼠死后组织细胞DNA含量变化与死亡时间的相关性研究 |
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| 作者姓名： | Liu ZP Chen X She YW |
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| 作者单位： | 1. 复旦大学上海医学院法医学系,上海,200002
2. 上海市刑事科学技术研究所,上海,200083 |
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| 基金项目： | 卫生部科研基金资助项目(98-1-169) |
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| 摘 要： | 目的应用流式细胞术研究大鼠死后组织细胞DNA含量变化与死亡时间的相关性。方法SD大鼠断颈处死后于不同时间段取心、肝、脾、肾器官组织,经胰蛋白酶消化等处理后制成单细胞悬液,应用流式细胞术,在620nm波长处检测各组不同器官组织细胞DNA含量,观察其变化规律。结果大鼠各器官组织细胞DNA含量随死亡时间延长均呈下降趋势。其中以脾组织细胞DNA含量变化趋势与死亡时间最具相关性,肝、肾次之,而心最差;死后48h,各器官组织细胞仅存微量完整的细胞核DNA。结论机体死亡后,各器官组织细胞核DNA含量随死亡时间的延长逐渐减少,具有一定的变化规律,可应用组织细胞DNA含量变化来推断死亡时间。
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| 关 键 词： | 死亡时间 DNA含量 Feulgen染色 流式细胞术 |
| 文章编号： | 1004-5619(2004)02-0068-02 |
| 修稿时间： | 2003年11月3日 |
Investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells of rat |
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| Liu ZP,Chen X,She YW.Investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells of rat[J].Journal of Forensic Medicine,2004,20(2):68-69. |
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| Authors: | Liu Zhi-ping Chen Xin She Yi-wen |
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| Institution: | Department of Forensic Medicine, Medical Center of Fudan University, Shanghai 200032, China. |
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| Abstract: | OBJECTIVE: To investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells. METHODS: After different PMI from the heart, liver, spleen and kidney were taken into pieces respectively, then centrifuged and digested to get suspending cells fluid. The amount of DNA of rats'viscera were detected by flow cytometry after stained by fluorescence, and also inspect the amount of DNA in different periods to find out the law of its variation. RESULTS: It showed a descendent trend of the amount of DNA in cells after different PMI, especially in spleen. CONCLUSION: The amount of DNA of all the viscera grows downwards after death, this might be applyed in forensic estimation of PMI. |
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| Keywords: | postmortem interval DNA degrade flow cytometry |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
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