| X染色体STR荧光复合扩增体系的建立及其应用 |
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| 引用本文： | 刘秋玲,吕德坚,赵虎.X染色体STR荧光复合扩增体系的建立及其应用[J].法医学杂志,2009,25(6):425-427. |
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| 作者姓名： | 刘秋玲 吕德坚 赵虎 |
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| 作者单位： | 中山大学,中山医学院法医学系,广东,广州,510080 |
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| 摘 要： | 目的研究X染色体STR在法医学亲权鉴定中的应用。方法利用荧光标记引物复合PCR技术,在同一反应管中同时检测DXS6801、DXS9902、DXS6809、DXS6803、DXS6804和DXS67996个X-STR基因座,采用3100遗传分析仪电泳和GeneMapper IDv 3.1软件进行基因分型。结果本体系同时分析6个X-STR基因座,结果清晰,灵敏度高,重复性好。结论本研究的6个X-STR基因座复合扩增体系,在法医学个体识别特别是女性的亲权鉴定中有重要应用价值。
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| 关 键 词： | 法医遗传学 同胞关系 染色体 人 X 荧光复合扩增 |
Development and Application of X -chromosomal STR Multiplex Amplification System |
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| LIU Qiu-ling,L De-jian,ZHAO Hu.Development and Application of X -chromosomal STR Multiplex Amplification System[J].Journal of Forensic Medicine,2009,25(6):425-427. |
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| Authors: | LIU Qiu-ling L De-jian ZHAO Hu |
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| Institution: | LIU Qiu-ling,L(U) De-jian,ZHAO Hu |
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| Abstract: | Objective To explore the application of X-chromosomal STR(X-STR) for forensic identification and paternity testing. Methods Six X-STR loci DXS6801, DXS9902, DXS6809, DXS6803, DXS6804 and DXS6799 were amplified in a single PCR reaction. PCR products were analyzed using capillary electrophoresis and 3100 Genetic Analyzer and GeneMapper ID v3.1 Analysis Software. Results The alleles of all six X-STR loci were successfully obtained with unambiguous genotyping, high sensitivity and reproducibility. Conclusion Th... |
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| Keywords: | forensic genetics sibling relations chromosomes human X fluorescent multiplex PCR |
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