首页 | 本学科首页   官方微博 | 高级检索  
     检索      

14株基因C型鸭甲肝病毒分离株的VP1基因变异及其编码蛋白的抗原表位分析
引用本文:皮晋魁,张焕容,汤承,岳华.14株基因C型鸭甲肝病毒分离株的VP1基因变异及其编码蛋白的抗原表位分析[J].中国兽医科学,2012(3):243-252.
作者姓名:皮晋魁  张焕容  汤承  岳华
作者单位:西南民族大学生命科学与技术学院;动物医学四川省高等学校重点实验室
基金项目:西南民族大学中央高校专项资金项目(11ZYXS22);西南民族大学中央高校基本科研业务费专项(82000466)
摘    要:对14株分离自四川不同地区的基因C型鸭甲肝病毒(DHAV-C)VP1基因进行了PCR扩增、测序、序列比对分析以及抗原表位分析。结果显示,14株DHAV-C VP1基因核苷酸序列的同源性为92.2%~100%,推导氨基酸序列的同源性为85.8%~100%,与GenBank中的24株DHAV-C VP1基因的核苷酸和氨基酸序列同源性分别为87.9%~99.3%和80.8%~100%。聚类分析显示,四川华阳地区分离的6株DHAV-C的VP1基因与黑龙江分离株Du/CH/LJS/090905同属一支,3株四川邛崃地区分离株与北京分离株C-GY及福建分离株FJ01同属一支,4株四川郫县分离株与1株四川邛崃分离株处于独立的小分支,它们与其他毒株之间存在一定的遗传距离。DHAV-C VP1蛋白由240个氨基酸组成,是由α螺旋、β折叠和无规则卷曲共同组成的混合型蛋白,VP1蛋白氨基酸序列的亲水性、抗原指数和表面可及性均较高,其中212~222位氨基酸区域的亲水性、抗原指数和表面可及性最高,可能是DHAV-C VP1蛋白上的B细胞表位优势区域,为DHAV-C表位疫苗的研究提供了理论依据。结果表明,四川分离株可能由于引种等原因从全国不同地区进入四川境内。

关 键 词:基因C型鸭甲肝病毒  VP1基因  同源性  抗原表位

Genetic heterogeneity in VP1gene and VP1 protein antigenic epitope of 14 strains of genotype C duck hepatitis A virus
PI Jin-kui,ZHANG Huan-rong,TANG Cheng,YUE Hua.Genetic heterogeneity in VP1gene and VP1 protein antigenic epitope of 14 strains of genotype C duck hepatitis A virus[J].Veterinary Science in China,2012(3):243-252.
Authors:PI Jin-kui  ZHANG Huan-rong  TANG Cheng  YUE Hua
Institution:1,2(1.College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China; 2.Sichuan Provincial Higher Education Key Laboratory of Veterinary Medicine,Chengdu 610041,China)
Abstract:Genetic heterogeneity of genotype C duck hepatitis A virus(DHAV-C) was investigated by direct amplification of VP1 gene of 14 DHAV-C isolates,which were obtained from different districts of Sichuan Province.The 14 DHAV-C isolates showed intragenotypic nucleotide and amino acid divergence with each other and with other strains in the GenBank.The nucleotide sequence homologies among the 14 DHAV-C isolates were 92.2%-100%,and amino acid sequence homologies were 85.8%-100%.The nucleotide sequence homologies among the 14 DHAV-C isolates and 24 DHAV-C strains in the GenBank were 87.9%-99.3%,and amino acid sequence homologies were 80.8%-100%.Cluster analysis showed that 6 DHAV-C isolates from Huayang district in Sichuan Province clustered together with the strain Du/CH/LJS/090905 from Heilongjiang Province,and three DHAV-C isolates from Qionglai districtclustered together with the strains C-GY from Beijing and FJ01 from Fujian Province.One isolate from Qionglai district and 4 from Pixian district in Sichuan Province clustered forming a separate branch.The DHAV-C protein was composed of 240 amino acids,included α helix,β overlaps and random coils.The hydrophilicity,antigen index and surface probability of DHAV-C VP1 protein were all high,and the highest position of that was 212-222 amino acid,which provided a theory basis for epitope vaccine research. This study indicated that the 14 DHAV-C isolated may come from different areas of China by importing breeds.
Keywords:genotype C duck hepatitis A virus(DHAV-C)  VP1 gene  homology  epitope
本文献已被 CNKI 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号