| 猕猴溶组织内阿米巴凝集素hgl基因LC3段的克隆及原核表达 |
| |
| 引用本文： | 余星明,姜波,杨光友,王强,余建秋,牛李丽,古小彬,邓家波,程安春,毕凤均,陈维刚.猕猴溶组织内阿米巴凝集素hgl基因LC3段的克隆及原核表达[J].中国兽医科学,2010,40(2). |
| |
| 作者姓名： | 余星明 姜波 杨光友 王强 余建秋 牛李丽 古小彬 邓家波 程安春 毕凤均 陈维刚 |
| |
| 作者单位： | 余星明,陈维刚,王强,余建秋,牛李丽,邓家波(成都动物园,四川,成都,610081);毕凤均(四川农业大学,实验动物中心,国家实验猕猴种源基地,四川,雅安,625014);姜波,杨光友,古小彬,程安春(四川农业大学,动物医学院,四川,雅安,625014) |
| |
| 基金项目： | 成都大熊猫繁育研究幕金项目，教育部"长江学者和创新团队发展计划"创新团队项目，国家科技基础条件平台项目 |
| |
| 摘 要： | 为了研究动物溶组织内阿米巴基因工程疫苗,以川西猕猴溶组织内阿米巴分离株中提取的总基因组DNA为模板,扩增了hgl基因抗原区LC3段,将其重组于原核表达载体pET-32b(+)中,构建了pET-32b(+)-hglLC3表达载体,转化至大肠杆菌BL21中,经IPTG诱导表达融合蛋白,获得了1200bp的hgl基因LC3编码区片段;SDS-PAGE分析结果显示,重组表达质粒产生了大小为66ku的目的条带,与预期的结果一致;Western-blot分析结果显示,重组蛋白与兔超免疫血清呈阳性反应,表明,该抗原可作为动物溶组织内阿米巴疫苗的候选抗原。
|
| 关 键 词： | 川西猕猴 溶组织内阿米巴 凝集素hgl基因 LC3段 原核表达 |
Cloning and prokaryotic expression of Gal/GalNAc lectin hgl LC3 gene of Entamoeba histolytica isolated from Macaca mulatta |
| |
| YU Xing-ming,JIANG Bo,YANG Guang-you,WANG Qiang,YU Jian-qiu,NIU Li-li,GU Xiao-bin,DENG Jia-bo,CHENG An-chun,BI Feng-jun,CHEN Wei-gang.Cloning and prokaryotic expression of Gal/GalNAc lectin hgl LC3 gene of Entamoeba histolytica isolated from Macaca mulatta[J].Veterinary Science in China,2010,40(2). |
| |
| Authors: | YU Xing-ming JIANG Bo YANG Guang-you WANG Qiang YU Jian-qiu NIU Li-li GU Xiao-bin DENG Jia-bo CHENG An-chun BI Feng-jun CHEN Wei-gang |
| |
| Institution: | YU Xing-ming1,JIANG Bo2,YANG Guang-you2,WANG Qiang1,YU Jian-qiu1,NIU Li-li1,GU Xiao-bin2,DENG Jia-bo1,CHENG An-chun2,BI Feng-jun3,CHEN Wei-gang1(1.Chengdu Zoo,Chengdu 610081,China,2.College of Veterinary Medicine,Sichuan Agricultural University,Ya\'an 625014,3.Experimental Animal Center,China) |
| |
| Abstract: | To develop genetic engineering vaccine against Entamoeba histolytica infection in animals,a LC3 segment of hgl gene of E.histolytica was amplified from total genomic DNA of E.histolytica from Macaca mulatta by RT-PCR,and then subcloned into the pET-32b(+) vector.The recombinant vector pET-32b(+)-hglLC3 was transformed into Escherichia coli BL21 for expression with IPTG induction.The LC3 segment of hgl gene of E.histolytica was 1 200 bp.SDS-PAGE analysis indicated that the expressed fusion protein in recombi... |
| |
| Keywords: | Macaca mulatta Entamoeba histolytica hgl gene LC3 segment prokaryotic expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
